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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Influence of environmental factors in the adherence of an atypical enteropathogenic Escherichia coli strain to epithelial cells

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Author(s):
Romao, Fabiano T. [1] ; Hernandes, Rodrigo T. [1, 2] ; Yamamoto, Denise [1] ; Osugui, Lika [1] ; Popi, Ana Flavia [1] ; Gomes, Tania A. T. [1]
Total Authors: 6
Affiliation:
[1] Univ Fed Sao Paulo UNIFESP, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, Sao Paulo - Brazil
[2] Univ Estadual Paulista UNESP, Dept Microbiol & Imunol, Inst Biociencias, Botucatu, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: BMC Microbiology; v. 14, DEC 20 2014.
Web of Science Citations: 3
Abstract

Background: Attachment is essential to maintain bacteria at their preferential intestinal colonization sites. There is little information on the influence of different environmental conditions in the interaction of atypical enteropathogenic Escherichia coli (aEPEC) strains with epithelial cells. In this study, we evaluated the effect of different glucose (5 and 25 mM) and CO2 (0.03 and 5%) concentrations and presence of bile salts on the adhesiveness of the aEPEC strain 1551-2. Results: We found that a CO2-enriched atmosphere enhanced the adhesiveness of the aEPEC 1551-2 strain independently of glucose concentrations or presence of bile salts. Conversely, the presence of high glucose concentration altered the original localized adherence (LA) pattern observed at 5 mM glucose, which is characterized by the formation of compact bacterial clusters, to a hybrid adherence pattern (LA and an aggregative adherence-like pattern). In addition, at high glucose concentration, there was increased expression of the fimA gene, which encodes the major subunit of type 1 pilus (T1P), and an isogenic fimA mutant displayed only LA. The presence of bile salts did not interfere with the adhesion properties of the 1551-2 strain to HeLa cells. Conclusions: Our data suggest that a CO2-enriched atmosphere could favor aEPEC adhesion to the host cells, whereas enhanced T1P production under high glucose concentration could allow bacteria to access more extensive intestinal colonization sites in the host at the beginning of the infectious process. (AU)

FAPESP's process: 11/12664-5 - Exploring the interactions of Enteropathogenic Escherichia coli with intestinal cells in vitro and in vivo
Grantee:Tânia Aparecida Tardelli Gomes do Amaral
Support type: Regular Research Grants