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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A microRNA signature profile in EBV+ diffuse large B-cell lymphoma of the elderly

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Author(s):
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de Andrade, Tathiana Azevedo [1] ; Evangelista, Adriane Feijo [2] ; Froes Campos, Antonio Hugo [3] ; Poles, Wagner Augusto [1] ; Borges, Natalia Morais [1] ; Coutinho Camillo, Claudia Malheiros [3] ; Soares, Fernando Augusto [3] ; Vassallo, Jose [3, 4] ; Paes, Roberto Pinto [5] ; Zerbini, Maria Claudia [6] ; Scapulatempo, Cristovam [2] ; Alves, Antonio Correa [1] ; Young, Ken H. [7] ; Braga Colleoni, Gisele Wally [1]
Total Authors: 14
Affiliation:
[1] Univ Fed Sao Paulo, UNIFESP, Sao Paulo - Brazil
[2] Hosp Canc Barretos, Barretos - Brazil
[3] AC Camargo Canc Ctr, Sao Paulo - Brazil
[4] Univ Estadual Campinas, Campinas, SP - Brazil
[5] Santa Casa Misericordia Sao Paulo, Sao Paulo - Brazil
[6] Univ Sao Paulo, Fac Med, Sao Paulo - Brazil
[7] Univ Texas MD Anderson Canc Ctr, Dept Hematopathol, Houston, TX 77030 - USA
Total Affiliations: 7
Document type: Journal article
Source: ONCOTARGET; v. 5, n. 23, p. 11813-11826, DEC 15 2014.
Web of Science Citations: 22
Abstract

Currently, there is no characteristic microRNA (miRNA) expression pattern in Epstein-Barr virus(+) diffuse large B-cell lymphoma of the elderly (EBV(+)DLBCLe). This study aims to characterize a signature profile and identify miRNAs that can be used as biomarkers and alternative therapeutic targets for EBV(+)DLBCLe. Seventy-one DLBCL patients aged 50 years and older were included and four EBV+ and four EBV- samples were analyzed in two miRNA array platforms (pilot study). A larger multicenter cohort (29 EBV(+)DLBCLe and 65 EBV-DLBCL patients) was used to validate the results by real-time polymerase chain reaction. In the pilot study, 9% of DLBCL were EBV(+)DLBCLe by in situ hybridization. In multicenter study, EBV(+)w DLBCLe group showed a predominance of non-germinal center B-cell origin. Overall survival duration of EBV(+)DLBCLe was significantly inferior to that of EBV-DLBCL patients. We found 10 deregulated miRNAs in the two groups, but only seven were statistically different. We confirmed overexpression of hsa-miR-126, hsa-miR-146a, hsa-miR-146b, hsa-miR-150, and hsa-miR-222 and underexpression of hsa-miR-151 in EBV(+)DLBCLe cases compared to EBV-DLBCL cases. Hsa-miR-146b and hsa-miR-222 showed high specificity for identifying EBV(+)DLBCLe. The present study proposed a miRNA signature for EBV(+)DLBCLe and our findings suggest that hsa-miR-146b and hsa-miR-222 could be biomarkers and therapeutic targets. (AU)

FAPESP's process: 10/17668-6 - Identification of biomarkers and possible therapeutical targets in B-cell lymphoproliferative disorders
Grantee:Gisele Wally Braga Colleoni
Support type: Research Projects - Thematic Grants