Antigenic enrichment of tumor cell lines: a strategy for personalized immunotherapeutic approaches.

Author(s)

Mariana Pereira Pinho

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas
• Defense date: 25 nov. 2014

Examining board members

Jose Alexandre Marzagao Barbuto; Niels Olsen Saraiva Câmara; Roger Chammas

• Advisor: Jose Alexandre Marzagao Barbuto

Abstract

Dendritic cells (DCs) are antigen presenting cells widely used in immunotherapy strategies, as the ones that utilize dendritic cell tumor cell hybrids. The present project evaluated the possibility of using cells from tumor cell lines, which were previously transfected with mRNA amplified from tumor cells against which a response is aimed, to fuse with DCs. The hybrids were able to induce a specific immune response. Also, it was possible to enriched one cell with antigens from another one, since transfected cells increased the amount of mRNAs from the donator cell, and expressed GFP when the donator cells expressed this protein. It was possible to successfully amplify the GFP specific mRNA. The mRNAs amplified from RNA of different cell lines contained mRNAs that were present in the total extracted RNA, but were not able to increase the expression of the molecules we attempted to detect in the transfected cells, showing that the protocol still need to be improved. Together, these results show that this new strategy is promising and deserves further investigation. (AU)

• FAPESP's process: 12/10939-0 - Antigenic enrichment of tumor cell lines: a strategy for personalized immunotherapeutic approaches
• Grantee: Mariana Pereira Pinho
• Support type: Scholarships in Brazil - Master