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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Virulence and pathogenicity of Candida albicans is enhanced in biofilms containing oral bacteria

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Author(s):
Cavalcanti, Yuri Wanderley [1, 2] ; Morse, Daniel James [1] ; da Silva, Wander Jose [2] ; Del-Bel-Cury, Altair Antoninha [2] ; Wei, Xiaoqing [1] ; Wilson, Melanie [1] ; Milward, Paul [1] ; Lewis, Michael [1] ; Bradshaw, David [1] ; Williams, David Wynne [1]
Total Authors: 10
Affiliation:
[1] Cardiff Univ, Sch Dent, Cardiff CF10 3AX, S Glam - Wales
[2] Univ Estadual Campinas, Piracicaba Dent Sch, Piracicaba, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: BIOFOULING; v. 31, n. 1, p. 27-38, 2015.
Web of Science Citations: 41
Abstract

This study examined the influence of bacteria on the virulence and pathogenicity of candidal biofilms. Mature biofilms (Candida albicans-only, bacteria-only, C. albicans with bacteria) were generated on acrylic and either analysed directly, or used to infect a reconstituted human oral epithelium (RHOE). Analyses included Candida hyphae enumeration and assessment of Candida virulence gene expression. Lactate dehydrogenase (LDH) activity and Candida tissue invasion following biofilm infection of the RHOE were also measured. Candida hyphae were more prevalent (p < 0.05) in acrylic biofilms also containing bacteria, with genes encoding secreted aspartyl-proteinases (SAP4/SAP6) and hyphal-wall protein (HWP1) up-regulated (p < 0.05). Candida adhesin genes (ALS3/EPA1), SAP6 and HWP1 were up-regulated in mixed-species biofilm infections of RHOE. Multi-species infections exhibited higher hyphal proportions (p < 0.05), up-regulation of IL-18, higher LDH activity and tissue invasion. As the presence of bacteria in acrylic biofilms promoted Candida virulence, consideration should be given to the bacterial component when managing denture biofilm associated candidoses. (AU)

FAPESP's process: 12/07436-6 - Analysis of the virulence of Candida albicans biofilms developed under different conditions
Grantee:Yuri Wanderley Cavalcanti
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)