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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A nanostructured genosensor for the early diagnosis of systemic arterial hypertension

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Rolim, Thalita [1] ; Cancino, Juliana [1] ; Zucolotto, Valtencir [1]
Total Authors: 3
[1] Univ Sao Paulo, Phys Inst Sao Carlos, Nanomed & Nanotoxicol Grp, BR-13560970 Sao Carlos, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: BIOMEDICAL MICRODEVICES; v. 17, n. 1 FEB 2015.
Web of Science Citations: 10

The rapid progress of nanomedicine, especially in areas related to medical imaging and diagnostics, has motivated the development of new nanomaterials that can be combined with biological materials for specific medical applications. One such area of research involves the detection of specific DNA sequences for the early diagnosis of genetic diseases, using nanoparticles-containing genosensors. Typical genosensors devices are based on the use of sensing electrodes -biorecognition platforms -containing immobilized capture DNA probes capable of hybridizing with specific target DNA sequences. In this paper we show that upon an appropriate design of the biorecognition platform, efficient sandwich-type genosensors based upon DNA-AuNPs nanocomplexes can be efficiently applied to the detection of a Systemic Arterial Hypertension (SAH) polymorphism located in intron 16 of the Angiotensin-converter enzyme (ACE) gene. Since SAH is intimately related to heart diseases, especially blood hypertension, its early detection is of great biomedical interest. The biorecognition platforms were assembled using mixed self-assembled monolayers (SAM(mix)), which provided the immobilization of organized architectures with molecular control. Detection of the DNA target sequence at concentrations down to 1 nM was carried out using electrochemical impedance spectroscopy (EIS). We show that the use of EIS combined with specific nanobiocomplexes represents an efficient method for the unambiguous detection of complementary DNA hybridization for preventative nanomedicine applications. (AU)

FAPESP's process: 10/14565-1 - Development of nanoparticles functionalized with oligonucleotides for applications as genosensors for hypertension
Grantee:Thalita Verônica Calheiros Rolim
Support type: Scholarships in Brazil - Master