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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Potential utility of melatonin as an antioxidant therapy in the management of sickle cell anemia

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Author(s):
Humberto da Silva, Danilo Gruenig [1, 2] ; Ricci, Jr., Octavio [3] ; de Almeida, Eduardo Alves [2] ; Bonini-Domingos, Claudia Regina [1]
Total Authors: 4
Affiliation:
[1] UNESP Sao Paulo State Univ, Dept Biol, Hemoglobin & Hematol Genet Dis Lab, BR-15054000 Sao Paulo - Brazil
[2] UNESP Sao Paulo State Univ, Dept Chem & Environm Sci, BR-15054000 Sao Paulo - Brazil
[3] FAMERP Sao Jose do Rio Preto Med Sch, Dept Med, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Pineal Research; v. 58, n. 2, p. 178-188, MAR 2015.
Web of Science Citations: 13
Abstract

This study aimed to assess antioxidant effects of melatonin treatment compared to N-acetylcysteine (NAC) and to their combination in a sickle cell suspension. Sickle erythrocytes were suspended in phosphate-buffered saline, pH 7.4, composing external control group. They were also suspended and incubated at 37 degrees C either in the absence (experimental control group) or in the presence of NAC, melatonin and their combination at concentrations of 100 pM, 100 nM and 100 mu M for 1 hr (treatment groups). The melatonin influences were evaluated by spectrophotometric {[}hemolysis degree, catalase (CAT), glutathione S-transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G6PDH), and superoxide dismutase (SOD) activities] and chromatographic methods {[}glutathione (GSH) and malondialdehyde (MDA) levels]. Incubation period was able to cause a rise about 64% on hemolysis degree as well as practically doubled the lipid peroxidation levels (P < 0.01). However, almost all antioxidants tested treatments neutralized this incubation effect observed in MDA levels. Among the antioxidant biomarkers evaluated, we observed a modulating effect of combined treatment on GPx and SOD activities (P < 0.01), which showed similar to 25% decrease in their activities. In addition, we found an antioxidant dose-dependent effect for melatonin on lipid peroxidation (r = -0.29; P = 0.03) and for combined antioxidant treatments also on MDA levels (r = -0.37; P = 0.01) and on SOD activity (r = -0.54; P < 0.01). Hence, these findings contribute with important insight that melatonin individually or in combination with NAC may be useful for sickle cell anemia management. (AU)

FAPESP's process: 13/07937-8 - Redoxome - Redox Processes in Biomedicine
Grantee:Ohara Augusto
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC