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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Rapid turbidimetric assay to potency evaluation of tigecycline in lyophilized powder

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Author(s):
da Silva, Lucelia Magalhaes [1] ; Nunes Salgado, Herida Regina [2]
Total Authors: 2
Affiliation:
[1] Univ Fed Alfenas UNIFAL, BR-37130000 Alfenas, MG - Brazil
[2] UNESP, Fac Ciencias Farmaceut Araraquara, Araraquara, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Microbiological Methods; v. 110, p. 49-53, MAR 2015.
Web of Science Citations: 2
Abstract

Tigecycline, a first-in-class glycylcycline and an analog of the semisynthetic antibiotic minocycline, is a potent, broad-spectrum antibiotic that acts by the inhibition of protein translation in bacteria. This glycylcycline inhibits Gram-positive, Gram-negative, atypical, anaerobic and antibiotic-resistant organisms. There is no microbiological analytical method for tigecycline in lyophilized powder reported yet. Thus, this paper reports the development and validation of a simple, sensitive, accurate and reproducible turbidimetric method to quantify tigecycline in lyophilized powder, using Staphylococcus aureus as microorganism test and 3 x 3 parallel line assay design, with twenty tubes for each assay. The validated method showed good results of linearity in the concentration range from 3 to 432 mu g/mL (r(2) = 0.9999), selectivity, precision, robustness and accuracy of 99.74%. The results demonstrated the validity of the proposed bioassay, which allows reliable quantitation of tigecydine in pharmaceutical samples and therefore can be used as a useful alternative methodology for the routine quality control of this medicine. (C) 2015 Elsevier B.V. All rights reserved. (AU)