| Full text | |
| Author(s): |
Mettifogo, Elena
[1]
;
Buzinhani, Melissa
[1]
;
Buim, Marcos R.
[2]
;
Timenetsky, Jorge
[1]
;
Piantino Ferreira, Antonio J.
[3]
Total Authors: 5
|
| Affiliation: | [1] Univ Sao Paulo, Dept Microbiol, Inst Ciencias Biomed ICBM, BR-05508270 Sao Paulo, SP - Brazil
[2] Inst Biol, BR-17690000 Bastos, SP - Brazil
[3] Univ Sao Paulo, Dept Patol, Fac Med Vet & Zootecnia, BR-05508000 Sao Paulo, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Pesquisa Veterinária Brasileira; v. 35, n. 1, p. 13-18, JAN 2015. |
| Web of Science Citations: | 0 |
| Abstract | |
Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are the mycoplasma infections of most concern for commercial poultry industry. MG infection is commonly designated as chronic respiratory disease (CRD) of chickens and infections sinusitis of turkeys. MS causes sub clinical upper respiratory infection and tenosynovitis or bursitis in chickens and turkeys. The multiplex PCR was standardized to detect simultaneously the MS, MG field strains and MG F-vaccine strain specific. The generic PCR for detection of any species of Mollicutes Class was performed and compared to the multiplex PCR and to PCR using species-specific primers. A total of 129 avian tracheal swabs were collected from broiler-breeders, layer hens and broilers in seven different farms and were examined by multiplex PCR methods. The system (multiplex PCR) demonstrated to be very rapid, sensitive, and specific. Therefore, the results showed a high prevalence of MS in the flocks examined (27.9%), and indicate that the MS is a recurrent pathogen in Brazilian commercial poultry flocks. (AU) | |