Lipoxygenase inhibitory activity of Cuspidaria pulchra and isolated compounds

Author(s)

Alvarenga, Tavane A. ^[1] ; Bertanha, Camila S. ^[1] ; de Oliveira, Pollyanna F. ^[1] ; Tavares, Denise C. ^[1] ; Gimenez, Valeria M. M. ^[2] ; Silva, Marcio L. A. ^[1] ; Cunha, Wilson R. ^[1] ; Januario, Ana H. ^[1] ; Pauletti, Patricia M. ^[1]

Affiliation

^[1] Univ Franca, Nucleo Pesquisa Ciencias Exatas & Tecnol, BR-14404600 Franca - Brazil

^[2] Ctr Univ Claretiano, BR-14300000 Sao Paulo - Brazil

• Document type: Journal article
• Source: NATURAL PRODUCT RESEARCH; v. 29, n. 11, p. 1083-1086, JUN 3 2015.
• Web of Science Citations: 1

Abstract

This work evaluated the in vitro inhibitory activity of the crude ethanolic extract from the aerial parts of Cuspidaria pulchra (Cham.) L.G. Lohmann against 15-lipoxygenase (15-LOX). The bioassay-guided fractionation of the n-butanol fraction, which displayed the highest activity, led to the isolation of three compounds: caffeoylcalleryanin (1), verbascoside (2) and 6-hydroxyluteolin-7-O-beta-glucoside (3). Assessment of the ability of the isolated compounds to inhibit 15-LOX revealed that compounds 1, 2 and 3 exerted strong 15-LOX inhibitory activity; IC50 values were 1.59, 1.76 and 2.35 mu M respectively. The XTT assay showed that none of the isolated compounds seemed to be significantly toxic. (AU)

• FAPESP's process: 13/09280-6 - Phytochemistry study and evaluation of the cytotoxicity and lipoxygenase inhibitory activity from Cerrado plants
• Grantee: Patricia Mendonça Pauletti
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/01139-7 - Chemical study of Cuspidaria pulchra (Cham.) L. G. Lohmann (Bignoniaceae) and evaluation of the Schistosomicidal activity.
• Grantee: Tavane Aparecida Alvarenga
• Support Opportunities: Scholarships in Brazil - Scientific Initiation