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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Mastoparan induces apoptosis in B1 6F10-Nex2 melanoma cells via the intrinsic mitochondrial pathway and displays antitumor activity in vivo

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Author(s):
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de Azevedo, Ricardo A. [1] ; Figueiredo, Carlos R. [2] ; Ferreira, Adilson K. [3] ; Matsuo, Alisson L. [2] ; Massaoka, Mariana H. [2] ; Girola, Natalia [2] ; Auada, Aline V. V. [1] ; Farias, Camyla F. [2] ; Pasqualoto, Kerly F. M. [1] ; Rodrigues, Cecilia P. [3] ; Barbuto, Jose A. [4] ; Levy, Debora [5] ; Bydlowski, Sergio P. [5] ; de Sa-Junior, Paulo L. [6] ; Travassos, Luiz R. [2] ; Lebrun, Ivo [1]
Total Authors: 16
Affiliation:
[1] Butantan Inst, Biochem & Biophys Lab, Sao Paulo, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Expt Oncol Unit UNONEX, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, BR-05508 Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Cell & Mol Therapy Ctr NUCEL NETCEM, Sao Paulo - Brazil
[5] Univ Sao Paulo, Sch Med, Lab Genet & Mol Hematol LIM31, BR-05508 Sao Paulo - Brazil
[6] Butantan Inst, Lab Genet, Sao Paulo, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Peptides; v. 68, p. 113-119, JUN 2015.
Web of Science Citations: 25
Abstract

Mastoparan is an a-helical and amphipathic tetradecapeptide obtained from the venom of the wasp Vespula lewisii. This peptide exhibits a wide variety of biological effects, including antimicrobial activity, increased histamine release from mast cells, induction of a potent mitochondrial permeability transition and tumor cell cytotoxicity. Here, the effects of mastoparan in malignant melanoma were studied using the murine model of B16F10-Nex2 cells. In vitro, mastoparan caused melanoma cell death by the mitochondrial apoptosis pathway, as evidenced by the Annexin V-FITC/PI assay, loss of mitochondrial membrane potential (Delta psi(m)), generation of reactive oxygen species, DNA degradation and cell death signaling. Most importantly, mastoparan reduced the growth of subcutaneous melanoma in syngeneic mice and increased their survival. The present results show that mastoparan induced caspase-dependent apoptosis in melanoma cells through the intrinsic mitochondrial pathway protecting the mice against tumor development. (C) 2014 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 10/51077-5 - Antitumor and apoptotic effects of peptides obtained from bovine b-casein INKKI and YPVPQFTE and its analogues and mastoparanes isolated from wasp venoms in experimental melanome models in vitro and in vivo
Grantee:Ivo Lebrun
Support type: Regular Research Grants