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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cardiolipin is a key determinant for mtDNA stability and segregation during mitochondrial stress

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Luevano-Martinez, Luis Alberto [1] ; Forni, Maria Fernanda [1] ; dos Santos, Valquiria Tiago [1] ; Souza-Pinto, Nadja C. [1] ; Kowaltowski, Alicia J. [1]
Total Authors: 5
[1] Univ Sao Paulo, Dept Bioquim, Inst Quim, BR-05508000 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS; v. 1847, n. 6-7, p. 587-598, JUN-JUL 2015.
Web of Science Citations: 17

Mitochondria play a key role in adaptation during stressing situations. Cardiolipin, the main anionic phospholipid in mitochondrial membranes, is expected to be a determinant in this adaptive mechanism since it modulates the activity of most membrane proteins. Here, we used Saccharomyces cerevisiae subjected to conditions that affect mitochondrial metabolism as a model to determine the possible role of cardiolipin in stress adaptation. Interestingly, we found that thermal stress promotes a 30% increase in the cardiolipin content and modifies the physical state of mitochondrial membranes. These changes have effects on mtDNA stability, adapting cells to thermal stress. Conversely, this effect is cardiolipin-dependent since a cardiolipin synthase-null mutant strain is unable to adapt to thermal stress as observed by a 60% increase of cells lacking mtDNA (rho(0)). Interestingly, we found that the loss of cardiolipin specifically affects the segregation of mtDNA to daughter cells, leading to a respiratory deficient phenotype after replication. We also provide evidence that mtDNA physically interacts with cardiolipin both in S. cerevisiae and in mammalian mitochondria. Overall, our results demonstrate that the mitochondrial lipid cardiolipin is a key determinant in the maintenance of mtDNA stability and segregation. (C) 2015 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 10/18254-0 - Molecular mechanisms of repair of double strand breaks in Mitochondrial DNA
Grantee:Valquiria Tiago dos Santos
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/04919-9 - Evaluation of the adaptive response of mitochondrial membranes to stress: role of cardiolipin in membrane plasticity, dynamics and pathological situations
Grantee:Luis Alberto Luevano Martinez
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 13/04871-6 - Effects of caloric restriction on mitochondrial morphology, dynamics, bioenergetics and redox state
Grantee:Maria Fernanda Pereira de Araújo Demonte Forni
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 13/07937-8 - Redoxome - Redox Processes in Biomedicine
Grantee:Ohara Augusto
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 10/51906-1 - Mitochondrial bioenergetics, ion transport, redox state and DNA metabolism
Grantee:Alicia Juliana Kowaltowski
Support type: Research Projects - Thematic Grants