| Full text | |
| Author(s): |
Total Authors: 2
|
| Affiliation: | [1] HCFMUSP, Inst Lab Invest Med, Sao Paulo - Brazil
[2] Inst Trop Med S Paulo, Protozool Lab, BR-05403000 Sao Paulo, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | JOURNAL OF IMMUNOLOGICAL METHODS; v. 420, p. 11-17, MAY 2015. |
| Web of Science Citations: | 0 |
| Abstract | |
Toxoplasma gondii infections are very common, causing occasional central nervous system and eye diseases, and must be screened in prenatal care for efficient therapy. Here, we developed a duplex solid-phase fluorescent assay (dFISA) for the simultaneous detection of anti-T. gondii IgG and IgM antibodies in prenatal care screening for toxoplasmosis. Assays using commercially available ion-exchange purified conjugates yielded poor results and high background fluorescence. Same-well IgG/IgM dFISA with refined conjugates was used to test 140 samples from university students, 120 samples from pregnant women and 24 samples from adult volunteers at a large public hospital. We found that dFISA offers high concordance, specificity and reproducibility for IgG (Kappa = 0.883) and IgM (Kappa = 0.918), which is useful in high-throughput applications for antenatal care. (C) 2015 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 13/04676-9 - Flow cytometry and fluorimetry in diseases caused by protozoa |
| Grantee: | Heitor Franco de Andrade Junior |
| Support Opportunities: | Regular Research Grants |