Using the comet and micronucleus assays for genotoxicity studies: A review

Author(s)

Araldi, Rodrigo Pinheiro ^{[1, 2, 3]} ; de Melo, Thatiana Correa ^{[2, 4]} ; Mendes, Thais Biude ^{[1, 4]} ; de Sa Junior, Paulo Luiz ^[2] ; Nakano Nozima, Bruno Heidi ^[4] ; Ito, Eliana Tiemi ^[1] ; de Carvalho, Rodrigo Franco ^[2] ; de Souza, Edislane Barreiros ^[1] ; Stocco, Rita de Cassia ^[2]

Affiliation

^[1] Univ Estadual Paulista Julio de Mesquita Filho UN, Fac Ciencias & Letras Assis, Lab Biol Mol Genet & Mutagenese, Sao Paulo - Brazil

^[2] Inst Butantan, Genet Lab, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Ciencias Biomed, Programa Posgrad Interunidades Biotecnol, BR-05508 Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo UNIFESP, Escola Paulista Med, Programa Posgrad Morfol Func & Estrutural, Sao Paulo - Brazil

• Document type: Review article
• Source: BIOMEDICINE & PHARMACOTHERAPY; v. 72, p. 74-82, MAY 2015.
• Web of Science Citations: 59

Abstract

Physical, chemical and biological agents can act in the DNA, resulting in mutation involved in cancer. Thus, genotoxic tests are required by regulatory agencies in order to evaluate potential risk of cancer. Among these tests, the comet assay (CA) and micronucleus assay (MNA) are the most commonly used. However, there are different protocols and recommendations already published. This is the first review, after the inclusion of CA in S2R1 guidance and OECD 489, which summarizes the main technical recommendations of both CA and MNA. (C) 2015 Published by Elsevier Masson SAS. (AU)

• FAPESP's process: 14/20617-5 - Study of the action of bovine papillomavirus (BPV) in epithelial-mesenchymal transition
• Grantee: Rodrigo Pinheiro Araldi
• Support Opportunities: Scholarships in Brazil - Doctorate