Cytokines, but not corticotropin-releasing factor and endothelin-1, participate centrally in the febrile response in zymosan-induced arthritis in rats

Author(s)

Kanashiro, Alexandre ^[1] ; Figueiredo, Maria J. ^[1] ; Malvar, David do C. ^[2] ; Souza, Gloria E. P. ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Phys & Chem, Pharmacol Lab, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Rural Rio de Janeiro, Dept Physiol Sci, Pharmacol Lab, BR-23890000 Seropedica, RJ - Brazil

• Document type: Journal article
• Source: Brain Research; v. 1610, p. 12-19, JUN 12 2015.
• Web of Science Citations: 3

Abstract

Recent literature has revealed that centrally generated prostaglandins participate in the febrile response in zymosan-induced arthritis in rats. However, it is not clear whether other centrally acting pyrogenic mediators such as cytokines, endothelins (ETs), and the corticotropin-releasing factor (CRF) contribute to the febrile response in this model. In the present study, rats were pretreated with intracerebroventricular (i.c.v.) injections of soluble TNF receptor I (sTNFRI), recombinant IL-1 receptor antagonist (IL-1ra), anti-rat IL-6 monoclonal antibody (AbIL-6), alpha-helical CRF9-41 (a nonselective CRF1/CRF2 receptor antagonist), BQ-123 (an ETA receptor antagonist), BQ-788 (an ETB receptor antagonist), and artificial cerebrospinal fluid (aCSF, control) prior to an intra-articular zymosan (4 mg) injection. Rectal temperatures were measured with a telethermometer. The administration of IL-1ra (200 mu g), sTNFRI (500 ng), and AbIL-6 (5 mu g) attenuated body temperature elevations after a zymosan injection. The administration of BQ-788 (3 pmol), BQ-123 (3 pmol), and alpha-helical CRF9-41 (25 mu g) did not affect the zymosan-induced febrile response. All the compounds used to pretreat the animals did not significantly alter their basal body temperatures. Together, the results here demonstrate that the febrile response in zymosan-induced arthritis in rats depends on the centrally acting pyrogenic cytokines TNF-alpha, IL-1 beta, and IL-6, but does not depend on either CRF or ET-1. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 08/09443-4 - Antipyretic effect of the main dipyrone metabolites on febrile response induced by LPS and Tityus serrulatus scorpion venom
• Grantee: David do Carmo Malvar
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/04237-2 - Antiinflammatory Cholinergic Pathway: The role of neuroimmunomodulation in the control of inflammatory response.
• Grantee: Alexandre Kanashiro
• Support Opportunities: Scholarships in Brazil - Young Researchers