Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Gill-specific (Na+, K+)-ATPase activity and alpha-subunit mRNA expression during low-salinity acclimation of the ornate blue crab Callinectes ornatus (Decapoda, Brachyura)

Full text
Author(s):
Leone, Francisco A. [1] ; Garcon, Daniela P. [1] ; Lucena, Malson N. [1] ; Faleiros, Rogerio O. [2, 3] ; Azevedo, Sergio V. [4] ; Pinto, Marcelo R. [1] ; McNamara, John C. [2, 3]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040901 Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14040901 Sao Paulo - Brazil
[3] Univ Sao Paulo, Ctr Biol Marinha, BR-14040901 Sao Paulo - Brazil
[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14040901 Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY; v. 186, p. 59-67, AUG 2015.
Web of Science Citations: 12
Abstract

We evaluate (Na+, K+)-ATPase activity, and protein and gene expression of the a-subunit in posterior gills 6 and 7 of Callinectes ornatus, a euryhaline crab, during a 10-day acclimation period from seawater (33 parts per thousand S) to low salinity (21 parts per thousand S). (Na+, K+)-ATPase activity decreased within 1 h after transfer to 21 parts per thousand S, values recovering by 24 h and attaining a maximum of approximate to 180 nmol Pi min(-1) mg(-1) after 10 days (approximate to 2.5-fold increase). (Na+, K+)-ATPase activity is approximate to 1.5-fold greater in gill 6 than in gill 7, independently of salinity. Relative expression of (Na+, K+)-ATPase alpha-subunit mRNA increased in both gills within 1- to 2-h exposure to low salinity, reaching an approximate to 8-fold maximum after 24-h exposure, decreasing slightly by 10 days acclimation to low salinity. This increase in alpha-subunit mRNA expression may underpin the increased (Na+, K+)-ATPase activity seen after 10 days acclimation to low salinity. Enzyme affinity for ATP was greater in gill 6 than in gill 7, in contrast to ouabain affinity that was greater in gill 7. Western blotting analysis identified a single immunoreactive band against the (Na+, K+)-ATPase a-subunit with an M-r of approximate to 105 kDa, independently of gill number and low salinity acclimation. Despite these differences, gills 6 and 7 appear to perform similar functions in salt uptake from the dilute medium. The partial cDNA sequence obtained for the gill (Na+, K+)-ATPase of C. ornatus (GenBank deposit KF056804) showed 97 to 91% identities with similar sequences from other portunid crab gills. The regulation of gill (Na+, K+)-ATPase activity during acclimation to low salinity is discussed. (C) 2015 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 13/24252-9 - The (Na+,K+)-ATPase in osmoregulatory capacity in crab Ucides cordatus: biochemical and molecular view
Grantee:Malson Neilson de Lucena
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 11/15776-9 - mRNA expression and ion transporter activity in fiddler crab (Uca) gills: does osmoregulatory capability underlie the occupation of distinct osmotic niches?
Grantee:Rogério Oliveira Faleiros
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 10/06395-9 - Role of (Na,K)-ATPase in osmorregulation and excretion of ammonium ions in euryhaline crustaceans: identification and influence of FXYD2 on the regulation of gill (Na,K)-ATPase of the crab Callinectes danae
Grantee:Daniela Pereira Garçon
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/22605-1 - Kinetic and molecular characterization of gill tissue (Na+,K+)-ATPase from Cardisoma guanhumi (Latreille)crab
Grantee:Francisco de Assis Leone
Support Opportunities: Regular Research Grants
FAPESP's process: 07/04859-5 - Insulin signaling pathway in caste varieties development in honey bees and labor division
Grantee:Sergio Vicente de Azevedo
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 10/17534-0 - Role of (Na,K)-ATPase in osmoregulation and excretion of ammonium ions in euryhaline crustaceans: identification and influence of FXYD2 on the regulation of gill (Na,K)-ATPase of the crab Callinectes danae
Grantee:Francisco de Assis Leone
Support Opportunities: Regular Research Grants