Molecular phylogeny and ultrastructure of Myxobolus cf. cuneus, a parasite of patinga hybrid and Henneguya pseudoplatystoma, a parasite of pintado hybrid

Author(s)

Milanin, Tiago ^[1] ; Mendes Maia, Antonio Augusto ^[2] ; Monteiro Silva, Marcia Ramos ^[2] ; Carriero, Mateus Maldonado ^[3] ; Adriano, Edson Aparecido ^{[4, 1]}

Affiliation

^[1] Univ Estadual Campinas, Dept Anim Biol, BR-13083970 Campinas, SP - Brazil

^[2] Univ Sao Paulo, Dept Vet Med, Fac Zootecnia & Engn Alimentos, BR-13635900 Pirassununga, SP - Brazil

^[3] Univ Fed Sao Carlos UFSCar, Dept Genet & Evolut, BR-13565905 Sao Carlos, SP - Brazil

^[4] Univ Fed Sao Paulo, Dept Biol Sci, BR-09972270 Diadema, SP - Brazil

• Document type: Journal article
• Source: ACTA PARASITOLOGICA; v. 60, n. 3, p. 442-450, SEP 2015.
• Web of Science Citations: 3

Abstract

Through morphological, histopathological and ultrastructural analysis of Myxobolus cuneus Adriano, Arana et Cordeiro, 2006 and Henneguya pseudoplatystoma Naldoni, Arana, Maia, Ceccarelli, Tavares, Borges, Pozo et Adriano, 2009 were identified infecting pacu respectively (Piaractus mesopotamicus) and hybrid pintado (Pseudoplatystoma corruscans x Pseudoplatystoma reticulatum) taken from Brazilian fish farms. The present study describes 18S rDNA sequencing of Myxobolus cf. cuneus infecting the spleen of farmed patinga, a hybrid fish resulting from the crossing of P. mesopotamicus x Piaractus brachypomus, and H. pseudoplatystoma found in farmed hybrid pintado from the state of Sao Paulo, Brazil. The study also provides new details of the host-parasite interface of M. cf. cuneus, which reveal that the plasmodial wall is composed of a single membrane connected to the plasmodium ectoplasm by numerous pinocytic canals. The plasmodia also displayed asynchronous development but had disporic pansporoblasts at different developmental stages; immature and mature spores were found at different depth levels of the plasmodium. Maximum likelihood phylogenetic analysis showed that M. cf. cuneus appeared as a sister species of Henneguya pellucida Adriano, Arana et Cordeiro, 2005 in a sub-clade composed mainly of myxosporean parasites of characiforms, and that H. pseudoplatystoma clustered in a sub-clade composed of Henneguya/Myxobolus spp. parasites of siluriform fish. (AU)

• FAPESP's process: 11/08549-6 - Evaluation of the involvement of invertebrate hosts in the life cycle of Myxozoa parasites of pintado (including hybrids) and pacu (including hybrids) from fish farm.
• Grantee: Tiago Milanin
• Support Opportunities: Scholarships in Brazil - Doctorate