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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In vivo evaluation of photodynamic inactivation using Photodithazine (R) against Candida albicans

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Author(s):
Carmello, J. C. [1] ; Dovigo, L. N. [2] ; Mima, E. G. [1] ; Jorge, J. H. [1] ; de Souza Costa, C. A. [3] ; Bagnato, V. S. [4] ; Pavarina, A. C. [1]
Total Authors: 7
Affiliation:
[1] Univ Estadual Paulista, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, BR-14801903 Araraquara, SP - Brazil
[2] Univ Estadual Paulista, Araraquara Dent Sch, Dept Social Dent, BR-14801903 Araraquara, SP - Brazil
[3] Univ Estadual Paulista, Araraquara Dent Sch, Dept Physiol & Pathol, BR-14801903 Araraquara, SP - Brazil
[4] Univ Sao Paulo, Phys Inst Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES; v. 14, n. 7, p. 1319-1328, 2015.
Web of Science Citations: 12
Abstract

This study describes the photoinactivation of Candida albicans in a murine model of oral candidosis, mediated by Photodithazine (R) (PDZ). Six-week-old female Swiss mice were immunosuppressed, and inoculated with C. albicans to induce oral candidosis. After five days, photodynamic inactivation (PDI) mediated by PDZ at concentrations of 75, 100, 125 and 150 mg L-1 was applied on the tongue of mice. Next, microbiological evaluation was performed by recovering C. albicans from the tongue via colony forming units (CFU mL(-1)). After 24 h of treatment, the animals were killed and the tongues were surgically removed for histological analysis. PDI was effective in reducing C. albicans on the tongue of mice using 100 mg L-1 of PDZ, when compared to the positive control group (without treatment). No adverse effect on the tongue tissue was verified after PDI. Therefore, PDI was effective for inactivation of C. albicans without causing any harmful effects on host tissues, which is promising for future clinical trials. (AU)

FAPESP's process: 08/00601-6 - Study of the effectiveness of antimicrobial photodynamic therapy on Candida inactivation and the intracelular damage caused in yeast cells. In vitro and in vivo evaluation of two photosensitizers.
Grantee:Lívia Nordi Dovigo
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
Grantee:Vanderlei Salvador Bagnato
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 08/03994-9 - Study of the effectiveness of antimicrobial photodynamic therapy on Candida inactivation and the intracelular damage caused in yeast cells: in vitro and in vivo evaluation of two photosensitizers
Grantee:Ana Cláudia Pavarina
Support Opportunities: Regular Research Grants