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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Investigation of the conformational flexibility of DGAT1 peptides using tryptophan fluorescence

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Author(s):
Lopes, Jose L. S. [1, 2] ; Araujo, Ana P. U. [1] ; Jameson, David M. [2]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Inst Phys Sao Carlos, BR-13560 Sao Carlos, SP - Brazil
[2] Univ Hawaii Manoa, Dept Cell & Mol Biol, Honolulu, HI 96822 - USA
Total Affiliations: 2
Document type: Journal article
Source: METHODS AND APPLICATIONS IN FLUORESCENCE; v. 3, n. 2 JUN 2015.
Web of Science Citations: 3
Abstract

The conformational behavior of synthetic peptides corresponding to the putative binding sites of the diacylglycerol acyltransferase 1 enzyme (a polytopic integral membrane protein) was investigated using steady-state and time-resolved fluorescence spectroscopies. Three small linear peptides with 13, 15 and 22 amino acid residues, containing one, two and three Trp residues, respectively, were studied in aqueous solution, in the absence and presence of model membranes. The high flexibility and unordered conformation of the peptides in solution were confirmed by the low Trp polarization values, the high accessibility to water-soluble quencher, and the fast rotational correlation times of the Trp residues. However, upon binding to the lipid systems, the Trp residues were incorporated within the acyl hydrophobic core and their lifetimes and rotational correlation times increased. Phasor plots were employed to analyze intensity decay of peptide-lipid binding and provided a trajectory, in phasor space, that lies along a line connecting the points of the free and bound peptide. This trajectory was analyzed to determine the association constant of the peptide to the model membrane. (AU)

FAPESP's process: 09/17698-5 - Structural investigation of the bovine enzyme diacylglyceroltransferase1 (DGAT1) and its interaction with lipid systems
Grantee:Jose Luiz de Souza Lopes
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/20414-4 - Modern advances in time-resolved fluorescence spectroscopy to investigate the binding of DGAT1 peptides to lipid substrates
Grantee:Jose Luiz de Souza Lopes
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 10/13036-5 - Study of the interaction between internal peptides from the bovine diacylglycerol acyltransferase 1 (DGAT1) and membrane models
Grantee:Nádia de Cássia Noronha
Support Opportunities: Scholarships in Brazil - Scientific Initiation