Role of homo-and heterofermentative lactic acid bacteria on hydrogen-producing reactors operated with cheese whey wastewater

Author(s)

Gomes, Bruna Carrer ^[1] ; Ferreira Rosa, Paula Rubia ^[2] ; Etchebehere, Claudia ^{[3, 4]} ; Silva, Edson Luiz ^[2] ; AmancioVaresche, Maria Bernadete ^[1]

Affiliation

^[1] Univ Sao Paulo, Engn Sch Sao Carlos EESC, Lab Biol Proc, Dept Hydraul & Sanitat, BR-13563120 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Chem Engn, BR-13565905 Sao Carlos, SP - Brazil

^[3] Univ Republica, Fac Ciencias, Montevideo - Uruguay

^[4] Univ Republica, Fac Quim, Inst Clemente Estable, Lab Ecol Microbiana, Microbiol, Montevideo - Uruguay

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY; v. 40, n. 28, p. 8650-8660, JUL 27 2015.
• Web of Science Citations: 12

Abstract

The prevalence of lactic acid bacteria (LAB) and the effects of their antimicrobial peptides on H-2 production in anaerobic fluidized bed reactors (AFBRs) operated with cheese whey (AFBR1 and AFBR2) were verified in this study. The AFBR1 received 5 g COD L-1 of cheese whey with decreasing hydraulic retention times (HRT) of 14 to 8 h. The AFBR2 was operated with 3-10 g COD L-1 of cheese whey with an HRT of 6 h. Next, 152 colonies were selected from de Man, Rogosa and Sharpe MRS agar plates, and 45 strains were classified as LAB. The counts oscillated between 6.6 and 8.1 log CFU mL(-1), indicating that the LAB survived and persisted in the AFBRs. Pure cultures were identified using 16S rRNA gene sequencing, and Lactococcus lactis was the prevalent LAB (70%) in both reactors. The highest H-2 yields (1.9 and 2.3 mol H-2 mol lactose(-1)) were obtained during the first operational phase in both reactors when a low organic loading rate (OLR) was applied, and when the growth of Lactococcus spp. was associated with Leuconostoc pseudomesenteroides. The bacteriocin-producing LAB (mostly Lactobacillus spp.) found on the specific phases of reactors AFBR1 and AFBR2 exerted a remarkable influence on H-2 yield. Copyright (C) 2015, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 09/15984-0 - Bioenergy production from wastewaters and environmental fitting of liquid and solid wastes generated
• Grantee: Marcelo Zaiat
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 12/20157-9 - Monitoring lactic acid bacteria in anaerobic reactors producing biohydrogen from whey milk
• Grantee: Bruna Carrer Gomes Malavazi
• Support Opportunities: Scholarships in Brazil - Post-Doctoral