| Full text | |
| Author(s): |
Borsanelli, Ana Carolina
[1]
;
Gaetti-Jardim Junior, Elerson
[2]
;
Doebereiner, Juergen
[3]
;
Dutra, Iveraldo S.
[4]
Total Authors: 4
|
| Affiliation: | [1] Univ Estadual Paulista Unesp, Programa Posgrad Med Vet, Fac Cencias Agr & Vet, BR-14664900 Jaboticabal, SP - Brazil
[2] UNESP, Dept Patol & Propedeut Clin, Fac Odontol Aracatuba, BR-16015050 Aracatuba, SP - Brazil
[3] Pesquisa Vet Brasileira, Empresa Brasileira Pesquisa Agr Embrapa, BR-23897970 Seropedica, RJ - Brazil
[4] UNESP, Fac Med Vet Aracatuba, Dept Apoio Prod & Saude Anim, BR-16050680 Aracatuba, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Pesquisa Veterinária Brasileira; v. 35, n. 3, p. 237-240, MAR 2015. |
| Web of Science Citations: | 8 |
| Abstract | |
Periodontitis in cattle is an infectious purulent progressive disease associated with strict anaerobic subgingival biofilm and is epidemiologically related to soil management at several locations of Brazil. This study aimed to detect Treponema species in periodontal pockets of cattle with lesions deeper than 5mm in the gingival sulcus of 6 to 24-month-old animals considered periodontally healthy. We used paper cones to collect the materials, after removal of supragingival plaques, and kept frozen (at -80°C) up to DNA extraction and polymerase chain reaction (PCR) using T. amylovorum, T. denticola, T. maltophilum, T. medium and T. vincentii primers. In periodontal pocket, it was possible to identify by PCR directly, the presence of Treponema amylovorum in 73% of animals (19/26), T. denticola in 42.3% (11/26) and T. maltophilum in 54% (14/26). Among the 25 healthy sites, it was possible to identify T. amylovorum in 18 (72%), T. denticola in two (8%) and T. maltophilum in eight (32%). Treponema medium and T. vincentii were not detected over all 51 evaluated samples. The presence of Treponema amylovorum, T. maltophilum and, in particular, the widely recognized T. denticola in subgingival microflora brings an original and potencially important contribution in studies of the bovine periodontitis. (AU) | |
| FAPESP's process: | 13/13701-7 - Genotyping of anaerobic bacteria isolated from bovine periodontitis lesions |
| Grantee: | Ana Carolina Borsanelli |
| Support Opportunities: | Scholarships in Brazil - Doctorate |