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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A Metalloproteomics Study on the Association of Mercury With Breast Milk in Samples From Lactating Women in the Amazon Region of Brazil

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Author(s):
dos Santos, Felipe Andre [1] ; Cavecci, Bruna [2] ; Souza Vieira, Jose Cavalcante [2] ; Franzini, Vanessa Pezza [3] ; Santos, Ademir [3] ; Leite, Aline de Lima [4] ; Rabelo Buzalaf, Marilia Afonso [4] ; Zara, Luiz Fabricio [5] ; Padilha, Pedro de Magalhaes [2]
Total Authors: 9
Affiliation:
[1] Fed Univ Mato Grosso Sul INQUI UFMS, Inst Chem, Campo Grande, MS - Brazil
[2] Sao Paulo State Univ IB UNESP, Inst Biosci, Dept Chem & Biochem, BR-18618970 Sao Paulo - Brazil
[3] Sao Paulo State Univ IB UNESP, Inst Chem, Dept Analyt Chem, BR-18618970 Sao Paulo - Brazil
[4] Univ Sao Paulo FOB USP, Bauru Dent Sch, Dept Biochem, Sao Paulo - Brazil
[5] Univ Brasilia UnB, Planaltina, DF - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Archives of Environmental Contamination and Toxicology; v. 69, n. 2, p. 223-229, AUG 2015.
Web of Science Citations: 8
Abstract

This study aimed to identify metalloproteins that lose their metal ions in the presence of mercury (Hg) and bind to Hg in breast milk samples collected from the riverine population of the Madeira River, a tributary of the Amazon River. Initially, total Hg was determined from the hair of lactating women to identify individuals who were contaminated followed by a proteomic analysis of breast milk samples through two-dimensional polyacrylamide gel electrophoresis after acetone precipitation. The presence of Hg in the obtained protein spots was determined by cold vapor atomic absorption spectrometry and cold vapor atomic fluorescence spectrometry. These determinations indicated the presence of Hg in one protein spot, which was then characterized through electrospray ionization tandem mass spectrometry. Based on searches in the UniProt database, this protein spot was identified as lysozyme C. (AU)

FAPESP's process: 13/21297-1 - Metallothioneins in fish from Amazon Region: metallomic study associated to mercury
Grantee:Pedro de Magalhães Padilha
Support type: Regular Research Grants
FAPESP's process: 10/51332-5 - Development of analytical methods for mercury metallomics studies in fish collected in the AHE JIRAU - Madeira River Basin area of influence
Grantee:Pedro de Magalhães Padilha
Support type: Regular Research Grants
FAPESP's process: 10/51506-3 - Mercury metallomics in breast milk from the riverside population of AHE JIRAU - Madeira River Basin area of influence
Grantee:Felipe André dos Santos
Support type: Scholarships in Brazil - Doctorate