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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Tissue apoptosis in mice infected with Leptospira interrogans serovar Icterohaemorrhagiae

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Author(s):
Marinho, Marcia [1] ; Taparo, Cilene Vidovix [1] ; Oliveira-Junior, Itamar S. [2] ; Venturoli Perri, Silvia Helena [1] ; Cardoso, Tereza Cristina [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Paulista, Sao Paulo State Univ, Sch Vet Med, Dept Support Prod & Anim Hlth, Aracatuba, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Inflammatory Mediators Anesthesiol Pain & In, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 21, JUL 28 2015.
Web of Science Citations: 1
Abstract

Background: This investigation aimed to evaluate the occurrence of some apoptotic features induced by Leptospira interrogans serovar Icterohaemorrhagiae infection in young BALB/c mice during 2, 4, 7, 10, 14 and 21 days post-infection (dpi). Methods: The animals were euthanized and lung, liver and kidneys were harvested to histopathology analysis and immunohistochemistry to caspase-3 antigen detection was performed. Results: Chromatin condensation in kidney and liver tissues, but not in lung tissue, was observed. Caspase-3 reactive cells, mainly characterized as renal epithelial cells, were detected in the days 14 and 21 at high levels when compared to days 2, 4 and 7 (p = 0.025; p < 0.05). Lung sections revealed caspase-3 labeled alveolar cells in 10 and 14 days post-infection was higher than observed at 7 days (p = 0.0497; p < 0.05). Liver sections demonstrated reactive cells at a highest level at 14 and 21 days post-infection when comparison to 2, 4, 7 and 10 days (p = 0.0069; p < 0.05). Conclusions: Our results suggest that infection of L. interrogans induce in kidney, liver and lung an activation of apoptosis mediated by caspase-3 dependent pathway in later phases of infectious process. (AU)