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| Full text | |
| Author(s): Show less - |
Marangoni, Karina
[1, 2]
;
Neves, Adriana F.
[3]
;
Rocha, Rafael M.
[4]
;
Faria, Paulo R.
[5]
;
Alves, Patricia T.
[1]
;
Souza, Aline G.
[1]
;
Fujimura, Patricia T.
[1]
;
Santos, Fabiana A. A.
[1]
;
Araujo, Thaise G.
[1]
;
Ward, Laura S.
[2]
;
Goulart, Luiz R.
[1, 6]
Total Authors: 11
|
| Affiliation: | [1] Univ Fed Uberlandia, Lab Nanobiotechnol, Inst Genet & Biochem, Uberlandia, MG - Brazil
[2] Univ Estadual Campinas, Lab Canc Mol Genet, Fac Med Sci, Campinas, SP - Brazil
[3] Univ Fed Goias, Inst Biotechnol, Mol Biol Lab, Catalao, GO - Brazil
[4] AC Camargo Canc Hosp, Sao Paulo, SP - Brazil
[5] Univ Fed Uberlandia, Inst Biomed Sci, Histol Lab, Uberlandia, MG - Brazil
[6] Univ Calif Davis, Dept Med Microbiol & Immunol, Davis, CA 95616 - USA
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 5, JUL 15 2015. |
| Web of Science Citations: | 11 |
| Abstract | |
We described the selection of a novel nucleic acid antibody-like prostate cancer (PCa) that specifically binds to the single-stranded DNA molecule from a 277-nt fragment that may have been partially paired and bound to the PCA3 RNA conformational structure. PCA3-277 aptamer ligands were obtained, and the best binding molecule, named CG3, was synthesized for validation. Aiming to prove its diagnostic utility, we used an apta-qPCR assay with CG3-aptamer conjugated to magnetic beads to capture PCA3 transcripts, which were amplified 97-fold and 7-fold higher than conventional qPCR in blood and tissue, respectively. Histopathologic analysis of 161 prostate biopsies arranged in a TMA and marked with biotin-labeled CG3-aptamer showed moderate staining in both cytoplasm and nucleus of PCa samples; in contrast, benign prostatic hyperplasia (BPH) samples presented strong nuclear staining (78% of the cases). No staining was observed in stromal cells. In addition, using an apta-qPCR, we demonstrated that CG3-aptamer specifically recognizes the conformational PCA3-277 molecule and at least three other transcript variants, indicating that long non-coding RNA (lncRNA) is processed after transcription. We suggest that CG3-aptamer may be a useful PCa diagnostic tool. In addition, this molecule may be used in drug design and drug delivery for PCa therapy. (AU) | |
| FAPESP's process: | 12/24911-0 - Application of RNA aptamers in diagnosis and therapy of prostate cancer |
| Grantee: | Karina Marangoni |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |