Lipopolysaccharide-induced Stem Cell Factor Messenger RNA Expression and Production in Odontoblast-like Cells

Author(s)

Penha Oliveira, Sandra Helena ^[1] ; Carvalho Santos, Vanessa Aparecida ^[2]

Affiliation

^[1] Univ Estadual Paulista, Dept Basic Sci, Pharmacol Lab, Sch Dent Aracatuba, BR-16018805 Aracatuba, SP - Brazil

^[2] Univ Estadual Paulista, Dept Pediat & Social Dent, Sch Dent Aracatuba, BR-16018805 Aracatuba, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF ENDODONTICS; v. 38, n. 5, p. 623-627, MAY 2012.
• Web of Science Citations: 3

Abstract

Introduction: This study was done to evaluate the mechanism involved in stem cell factor (SCF) expression and production by lipopolysaccharide (LPS)-stimulated odontoblast (OD)-like cells and to investigate the signal transduction pathway activated in the process. Methods: ODs-like cells (MDPC-23) were stimulated with different LPS concentrations for 1, 6, and 24 hours. SCF expression in OD-like cells was analyzed by reverse-transcriptase polymerase chain reaction, and SCF production was assessed by enzyme-linked immunosorbent assay. In another set of experiments, OD-like cells were pretreated with dexamethasone (DEX), MK886 (MK), p42/44 inhibitor (PD 98059 {[}PD]), p38 inhibitor (SB 202190 {[}SB]), or PI3K inhibitor (wortmannin {[}Wort]) for 30 minutes followed by stimulation with LPS (0.1 mu g/mL) for 1 hour. Results: OD-like cells stimulated with LPS (0.1 mu g/mL) for 1 hour expressed SCF, but SCF production decreased with increasing LPS concentrations (1, 10, and 100 mu g/mL). DEX and MK were able to inhibit SCF messenger RNA (mRNA) expression. PD, SB, and Wort inhibited the SCF mRNA expression. Conclusions: LPS-induced SCF mRNA expression and production in OD-like cells occur via leukotriene production or cytokine and/or chemokine formation, activating the p42/44, p38, and PI3K pathways. Data suggest that SCF released by OD-like cells may act as immune response modulators. (J Endod 2012;38:623-627) (AU)