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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Revisiting the taxonomical classification of Porcine Circovirus type 2 (PCV2): still a real challenge

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Author(s):
Franzo, Giovanni [1] ; Cortey, Marti [2] ; Olvera, Alex [3] ; Novosel, Dinko [4] ; Martins Gomes De Castro, Alessandra Marnie [5] ; Biagini, Philippe [6] ; Segales, Joaquim [7, 8] ; Drigo, Michele [1]
Total Authors: 8
Affiliation:
[1] Univ Padua, Dept Anim Med Prod & Hlth MAPS, I-35020 Legnaro, PD - Italy
[2] Pirbright Inst, Woking, Surrey - England
[3] IrsiCaixa HIVACAT, Badalona - Spain
[4] Croatian Vet Inst, Dept Pathol, Zagreb - Croatia
[5] Univ Sao Paulo, Coll Vet Med, Dept Prevent Vet Med & Anim Hlth, Brazil Coll Vet Med, Sao Paulo - Brazil
[6] CNRS, Unite Virol Mol Emergence & Coevolut Viale UMR 72, Marseille - France
[7] UAB, Ctr Recerca Sanitat Anim CReSA, IRTA UAB, Bellaterra 08193 - Spain
[8] Univ Autonoma Barcelona, Dept Sanitat & Anat Anim, Bellaterra 08193 - Spain
Total Affiliations: 8
Document type: Journal article
Source: VIROLOGY JOURNAL; v. 12, AUG 28 2015.
Web of Science Citations: 43
Abstract

Background: PCV2 has emerged as one of the most devastating viral infections of swine farming, causing a relevant economic impact due to direct losses and control strategies expenses. Epidemiological and experimental studies have evidenced that genetic diversity is potentially affecting the virulence of PVC2. The growing number of PCV2 complete genomes and partial sequences available at GenBank questioned the accepted PCV2 classification. Methods: Nine hundred seventy five PCV2 complete genomes and 1,270 ORF2 sequences available from GenBank were subjected to recombination, PASC and phylogenetic analyses and results were used for comparison with previous classification scheme. Results: The outcome of these analyses favors the recognition of four genotypes on the basis of ORF2 sequences, namely PCV2a, PCV2b, PCV2c and PCV2d-mPCV2b. To deal with the difficulty of founding an unambiguous classification and accounting the impossibility to define a p-distance cut-off, a set of reference sequences that could be used in further phylogenetic studies for PCV2 genotyping was established. Being aware that extensive phylogenetic analyses are time-consuming and often impracticable during routine diagnostic activity, ORF2 nucleotide positions adequately conserved in the reference sequences were identified and reported to allow a quick genotype differentiation. Conclusions: Globally, the present work provides an updated scenario of PCV2 genotypes distribution and, based on the limits of the previous classification criteria, proposes new rapid and effective schemes for differentiating the four defined PCV2 genotypes. (AU)