| Full text | |
| Author(s): |
Camargo, Roberto de A.
[1, 2]
;
Herai, Roberto H.
[3, 4]
;
Santos, Luana N.
[5]
;
Bento, Flavia M. M.
[1, 2]
;
Lima, Joni E.
[1]
;
Marques-Souza, Henrique
[5]
;
Figueira, Antonio
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Ctr Energia Nucl Agr, BR-13400970 Piracicaba, SP - Brazil
[2] Univ Sao Paulo, Escola Super Agr Luiz Queiroz ESALQ, BR-13418900 Piracicaba, SP - Brazil
[3] Univ Calif San Diego, Sch Med, Dept Pediat Cellular & Mol Med, La Jolla, CA 92093 - USA
[4] Pontificia Univ Catolica Parana, Sch Med, Grad Program Hlth Sci, BR-80215901 Curitiba, PR - Brazil
[5] Univ Estadual Campinas, Inst Biol, Dept Histologia & Embriol, BR-13083863 Campinas, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | BMC Genomics; v. 16, AUG 26 2015. |
| Web of Science Citations: | 7 |
| Abstract | |
Background: Providing double-stranded RNA (dsRNA) to insects has been proven to silence target genes, and this approach has emerged as a potential method to control agricultural pests by engineering plants to express insect dsRNAs. A critical step of this technology is the screening of effective target genes essential for insect development and/or survival. The tomato leafminer (Tuta absoluta Meyrick) is a major Solanum lycopersicum (tomato) pest that causes significant yield losses and has recently invaded Europe, from where it is spreading at an alarming rate. To explore RNA interference (RNAi) against T. absoluta, sequence information on potential target genes is necessary, but only a few sequences are available in public databases. Results: We sequenced six libraries from RNA samples from eggs, adults, and larvae at four stages, obtaining an overall total of around 245 million reads. The assembled T. absoluta transcriptome contained 93,477 contigs with an average size of 1,574 bp, 59.8 % of which presented positive Blast hits, with 19,995 (21.4 %) annotated by gene ontology. From the transcriptome, most of the core genes of the RNAi mechanism of Lepidoptera were identified indicating the potential suitability of T. absoluta for gene silencing. No contigs displayed significant similarity with a RNA-dependent RNA Polymerase. Genes from the juvenile hormone and ecdysteroid biosynthetic pathways were identified, representing potential target genes for systemic silencing. Comparisons of transcript profiles among stages revealed 1,577 genes differentially expressed at earlier larval stages, from which potential gene targets were identified. Five of these genes were evaluated using in vitro transcribed dsRNA absorbed by tomato leaflets, which were fed to 1st instar T. absoluta larvae, resulting in significant reduction of larval body weight while exhibiting significant knockdown for three of the genes. Conclusions: The transcriptome we generated represents a valuable genomic resource for screening potential gene targets that affect the development or survival of T. absoluta larvae. Five novel genes that showed greater expression at the 1st larval stage were demonstrated to be effective potential RNAi targets by reducing larval weight and can be considered good candidates for use in RNAi-mediated crop protection. (AU) | |
| FAPESP's process: | 10/11313-1 - Identification, physiological characterization and gene expression profilling of sugarcane (Saccharum spp.) cultivars contrasting for nitrogen use efficiency |
| Grantee: | Joni Esrom Lima |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 11/12869-6 - RNAi to control Tuta absoluta in tomato |
| Grantee: | Antonio Vargas de Oliveira Figueira |
| Support Opportunities: | Regular Research Grants |