Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of estradiol, progestogens, and of tibolone on breast proliferation and apoptosis

Full text
Author(s):
Pompei, L. M. [1] ; Cunha, E. P. [1] ; Steiner, M. L. [1] ; Theodoro, T. R. [2] ; Mader, A. M. A. A. [3] ; Petri, G. [4] ; Pinhal, M. A. S. [2] ; Fernandes, C. E. [1]
Total Authors: 8
Affiliation:
[1] Fac Med ABC, Discipline Gynecol, Santo Andro - Brazil
[2] Fac Med ABC, Discipline Biochem, Santo Andro - Brazil
[3] Fac Med ABC, Discipline Pathol, Santo Andro - Brazil
[4] Fac Med ABC, Vivarium, Santo Andro - Brazil
Total Affiliations: 4
Document type: Journal article
Source: CLIMACTERIC; v. 18, n. 4, p. 518-522, AUG 2015.
Web of Science Citations: 0
Abstract

Aim To study the effects of estrogen therapy, alone or combined with progestogens, and of tibolone on the expression of proliferation and apoptosis markers in normal breast tissue. Methods Thirty 250-day-old Wistar rats were castrated and 3 weeks later received one of the following treatments by gavage for 5 weeks: (1) estradiol benzoate; (2) estradiol benzoate + medroxyprogesterone acetate; (3) estradiol benzoate + norethisterone acetate; (4) estradiol benzoate + dydrogesterone; (5) tibolone; (6) placebo. Following treatment, the expression of proliferating cell nuclear antigen (PCNA) and caspase-3 was analyzed by quantitative immunohistochemistry in the breast tissue, and proliferation and apoptosis were analyzed semiquantitatively by microscopic imaging. Results There was a statistically significant difference among the groups for PCNA, caspase-3 and the caspase-3 : PCNA ratio. Tibolone was associated with the lowest proliferative activity, followed by estradiol benzoate + dydrogesterone; however, estradiol benzoate + dydrogesterone showed the greatest rate of apoptosis. Conclusions The various progestogens can have more or less proliferative and pro-apoptotic effects than estradiol alone. Among the treatment schemes analyzed, the estradiol + dydrogesterone combination resulted in a higher apoptosis rate in relation to the proliferation rate and tibolone was associated with the lowest proliferation. (AU)

FAPESP's process: 11/13704-0 - Effect of tibolone, estradiol and estradiol combined with medroxyprogesterone acetate or norethindrone acetate or micronized progesterone in the presence of vascular matrix metalloproteinases and histomorphometric changes in breast tissue of rats
Grantee:Luciano de Melo Pompei
Support Opportunities: Regular Research Grants