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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Microvascularization of corpus luteum of bovine treated with equine chorionic gonadotropin

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Author(s):
Bezerra Moura, Carlos Eduardo [1] ; Rigoglio, Nathia Nathaly [2] ; Braz, Janine Karla Franca S. [3] ; Machado, Marcello [4] ; Baruselli, Pietro S. [5] ; Papa, Paula De Carvalho [2]
Total Authors: 6
Affiliation:
[1] Fed Rural Univ Semiarid Reg UFERSA, Dept Anim Sci, BR-59625900 Mossoro, RN - Brazil
[2] Univ Sao Paulo, Dept Surg, Sch Vet Med & Anim Sci, BR-05508270 Sao Paulo, SP - Brazil
[3] Fed Univ Rio Grande Norte UFRN, Dept Morphol, Biosci Ctr, BR-59078970 Natal, RN - Brazil
[4] Univ Santa Catarina, Dept Biol & Vet Sci, BR-89529000 Curitibanos, SC - Brazil
[5] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Sao Paulo, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: MICROSCOPY RESEARCH AND TECHNIQUE; v. 78, n. 9, p. 747-753, SEP 2015.
Web of Science Citations: 3
Abstract

This study aimed to evaluate the morphological changes in microvascular density and corpus luteum (CL) vascularization in cows treated with eCG during stimulatory and superovulatory protocols. Sixteen cows were synchronized and divided into three groups: control (n=6), stimulated (n=4) and superovulated (n =6), one was submitted to estrous synchronization (ES) and received no eCG (control), and those that were submitted to ES and received eCG before or after follicular deviation (superovulation and stimulation of the dominant follicle, respectively). Ovulation was synchronized using a progesterone device-based protocol. After six days of ovulation, the cows were slaughtered and the ovaries and CL were collected. The CLs were processed and photomicrographs were taken under light microscopy to assess the vascular volume density (Vv) by stereology, and scanning electron microscopy (SEM) was used to perform ultrastructural analysis of the microvasculature. The Vv in stimulated and superovulated cows significantly increased (P0.0001) when compared to control, indicating that the eCG is able to induce angiogenic activity in bovine CL. However, no significant differences were observed between stimulated and superovulated cows. The SEM demonstrated ratings indicative of angiogenesis, marked by several button-shaped projections in the capillaries, and the presence of more dilated capillaries in CL treated with eCG. These morphological findings are evidence of an angiogenic effect of the eCG treatment in CL of cows. Microsc. Res. Tech. 78:747-753, 2015. (c) 2015 Wiley Periodicals, Inc. (AU)