| Full text | |
| Author(s): |
Costa, C. A. R. A.
[1]
;
Tanimoto, A.
[1]
;
Quaglio, A. E. V.
[1]
;
Almeida, Jr., L. D.
[1]
;
Severi, J. A.
[1]
;
Di Stasi, L. C.
[1]
Total Authors: 6
|
| Affiliation: | [1] Univ Estadual Paulista, UNESP, Lab Phytomed Pharmacol & Biotechnol PhytoPharmaTe, Dept Pharmacol, Inst Biosci, BR-18618970 Botucatu, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | International Immunopharmacology; v. 28, n. 1, p. 459-469, SEP 2015. |
| Web of Science Citations: | 4 |
| Abstract | |
Inflammatory bowel disease (IBD) is a chronic, relapsing, idiopathic inflammation of the gastrointestinal tract. Clinical studies suggest that the initiation of IBD is multifactorial, involving genetics, the immune system and environmental factors, such as diet, drugs and stress. Pfaffia paniculata is an adaptogenic medicinal plant used in Brazilian folk medicine as an ``anti-stress{''} agent. Thus, we hypothesised that the P. paniculata enhances the response of animals subjected to colonic inflammation. Our aim was to investigate the intestinal anti-inflammatory activity of P. paniculata in rats before or after induction of intestinal inflammation using trinitrobenzenesulfonic add (TNBS). The animals were divided into groups that received the vehicle, prednisolone or P. paniculata extract daily starting 14 days before or 7 days after TNBS induction. At the end of the procedure, the animals were killed and their colons were assessed for the macroscopic damage score (MDS), extent of the lesion (EL) and weight/length ratio, myeloperoxidase (MPO) activity and glutathione (GSH), cytokines and Creactive protein (CRP) levels. Histological evaluation and ultrastructural analysis of the colonic samples were performed. Treatment with the 200 mg/kg dose on the curative schedule was able to reduce the MDS and the EL In addition, MPO activity was reduced, GSH levels were maintained, and the levels of pro-inflammatory cytokines and CRP were decreased. In conclusion, the protective effect of P. paniculata was related to reduced oxidative stress and CRP colonic levels, and due to immunomodulatory activity as evidenced by reduced levels of IL-1 beta, INF-gamma, TNF-alpha and IL-6. (C) 2015 Elsevier B.V. All rights reserved. (AU) | |