ANXA1(Ac2-26) peptide reduces ID1 expression in cervical carcinoma cultures

Author(s)

Prates, Janesly ^[1] ; Franco-Salla, Gabriela Bueno ^[1] ; Dinarte dos Santos, Anemari Ramos ^[2] ; da Silva, Jr., Wilson Araujo ^[2] ; da Cunha, Bianca Rodrigues ^[3] ; Tajara, Eloiza Helena ^[3] ; Oliani, Sonia Maria ^[1] ; Rodrigues-Lisoni, Flavia Cristina ^[4]

Affiliation

^[1] UNESP, Dept Biol, Inst Biosci Letters & Sci, IBILCE, Sao Jose Do Rio Preto, SP - Brazil

^[2] Univ Sao Paulo, FCFRP, Fac Med Ribeirao Preto, Dept Clin Med, Fdn Blood Ctr Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[3] FAMERP, Biol Fac Med Sao Jose do Rio Preto, Dept Mol, Sao Jose Do Rio Preto, SP - Brazil

^[4] Univ Estadual Paulista, FEIS, Dept Biol & Anim Sci, BR-15385000 Sao Paulo - Brazil

• Document type: Journal article
• Source: Gene; v. 570, n. 2, p. 248-254, OCT 10 2015.
• Web of Science Citations: 5

Abstract

Cervical cancer is the second most frequent cancer in women worldwide and is associated with genetic alterations, infection with human papilloma virus (HPV), angiogenesis and inflammatory processes. The idea that inflammation is involved in tumorigenesis is supported by the frequent appearance of cancer in areas of chronic inflammation. On the other hand, the inflammatory response is controlled by the action of anti-inflammatory mediators, among these mediators, annexin A1 (ANXA1), a 37 kDa protein was detected as a modulator of inflammatory processes and is expressed by tumor cells. The study was carried out on the epithelial cancer cell line (SiHa) treated with the peptide of annexin Al (ANXA1(Ac2-26)). We combined subtraction hybridization approach, Ingenuity Systems software and quantitative PCR, in order to evaluate gene expression influenced by ANXA1. We observed that ANXA1(Ac2-26) inhibited proliferation in SiHa cells after 72 h. In these cells, 55 genes exhibited changes in expression levels in response to peptide treatment. Six genes were selected and the expression results of 5 up-regulated genes (TPT1, LDHA, NCOA3, H1F1A, RAB13) and one down-regulated gene (ID1) were research by real time quantitative PCR. Four more genes (BMP4, BMPR1B, SMAD1 and SMAD4) of the ID1 pathway were investigated and only one (BMPR1B) shows the same down regulation. The data indicate the involvement of ANXA1(Ac2-26) in the altered expression of genes involved in tumorigenic processes, which could potentially be applied as a therapeutic indicator of cervical cancer. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 12/08320-1 - Genomic analysis of the cervical carcinoma cells treated with the anti-inflammatory protein annexin A1
• Grantee: Flávia Cristina Rodrigues Lisoni
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/08177-4 - Investigation of the differential genetic expression in reply to the effect of the anti-inflammatory protein Anexina A1 in the cervical carcinoma
• Grantee: Janesly Prates
• Support Opportunities: Scholarships in Brazil - Master