MICROSATELLITE MARKERS FOR STUDIES WITH THE CARNIVOROUS PLANT PHILCOXIA MINENSIS (PLANTAGINACEAE)

Author(s)

Scatigna, Andre V. ^[1] ; Oliveira, Fernanda A. ^[2] ; Mantello, Camila C. ^[2] ; Francisco, Patricia M. ^[2] ; Souza, Anete P. ^{[2, 1]} ; Simoes, Andre O. ^[1]

Affiliation

^[1] Univ Estadual Campinas UNICAMP, Dept Biol Vegetal, Sao Paulo - Brazil

^[2] Univ Estadual Campinas UNICAMP, Ctr Biol Mol & Engn Genet, Sao Paulo - Brazil

• Document type: Journal article
• Source: APPLICATIONS IN PLANT SCIENCES; v. 3, n. 8 AUG 2015.
• Web of Science Citations: 3

Abstract

Premise of the study: Microsatellite markers were developed for the critically endangered carnivorous species Philcoxia minensis (Plantaginaceae) for further population genetic studies aiming at its conservation. Methods and Results: We identified 29 clones containing 40 microsatellites from a genomic enriched library. A total of 27 primer pairs were developed and evaluated in 30 individuals of a natural P. minensis population. Seventeen markers successfully presented amplification products within the expected size range, of which 12 were polymorphic. The expected and observed heterozygosities ranged from 0.03 to 0.65 and from 0.00 to 0.77, respectively. Positive transferability with the related species P. bahiensis was observed for the same 17 markers. Conclusions: The 12 polymorphic microsatellite markers are suitable for studies in genetic diversity and structure, mating system, and gene flow in P. minensis and also may be useful for similar issues regarding the related species P. bahiensis. (AU)

• FAPESP's process: 12/12927-9 - Ecology and evolution of Philcoxia P. Taylor & V.C. Souza (Plantaginaceae)
• Grantee: André Olmos Simões
• Support Opportunities: Regular Research Grants