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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

MCT1 and MCT4 Kinetic of mRNA Expression in Different Tissues After Aerobic Exercise at Maximal Lactate Steady State Workload

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Author(s):
de Araujo, G. G. [1, 2, 3] ; Gobatto, C. A. [1] ; de Barros Manchado-Gobatto, F. [1] ; Teixeira, L. F. M. [4] ; dos Reis, I. G. M. [1] ; Caperuto, L. C. [5] ; Papoti, M. [6] ; Bordin, S. [5] ; Cavaglieri, C. R. [4] ; Verlengia, R. [4]
Total Authors: 10
Affiliation:
[1] Campinas State Univ UNICAMP, Sch Appl Sci, Lab Sport Appl Physiol, Sao Paulo - Brazil
[2] Fed Univ Alagoas UFAL, Phys Educ CEDU, Sports Sci Res Grp, Maceio, Alagoas - Brazil
[3] Fed Univ Alagoas UFAL, Post Grad Nutr, Maceio, Alagoas - Brazil
[4] Univ Metodista Piracicaba, Fac Hlth Sci, Phys Educ, Sao Paulo - Brazil
[5] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo - Brazil
[6] Univ Sao Paulo, Phys Educ Sch Ribeirao Preto, Sao Paulo - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Physiological Research; v. 64, n. 4, p. 513-522, 2015.
Web of Science Citations: 5
Abstract

We evaluate the mRNA expression of monocarboxylate transporters 1 and 4 (MCT1 and MCT4) in skeletal muscle (soleus, red and white gastrocnemius), heart and liver tissues in mice submitted to a single bout of swimming exercise at the maximal lactate steady state workload (MLSSw). After 72 h of MLSS test, the animals were submitted to a swimming exercise session for 25 min at individual MLSSw. Tissues and muscle samples were obtained at rest (control, n=5), immediately (n=5), 5 h (n=5) and 10 h (n=5) after exercise for determination of the MCT1 and MCT4 mRNA expression (RT-PCR). The MCT1 mRNA expression in liver increased after 10 h in relation to the control, immediate and 5 h groups, but the MCT4 remained unchanged. The MCT1 mRNA expression in heart increased by 31 % after 10 h when compared to immediate, but no differences were observed in relation to the control group. No significant differences were observed for red gastrocnemius in MCT1 and MCT4 mRNA expression. However, white gastrocnemius increased MCT1 mRNA expression immediately when compared to rest, 5 and 10 h test groups. In soleus muscle, the MCT1 mRNA expression increased immediately, 5 and 10 h after exercise when compared to the control. In relation to MCT4 mRNA expression, the soleus increased immediately and 10 h after acute exercise when compared to the control group. The soleus, liver and heart were the main tissues that showed improved the MCT1 mRNA expression, indicating its important role in controlling MLSS concentration in mice. (AU)