| Full text | |
| Author(s): |
Aguiar, Juliana Martins
[1]
;
da Silva, Sheila Oliveira
[1]
;
dos Santos, Valdir Antonio
[2]
;
Razera Rossa, Giselle Ayres
[1]
;
Taniwaki, Sueli Akemi
[1]
;
Gregori, Fabio
[1]
;
Keid, Lara Borges
[3]
;
Soares, Rodrigo Martins
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Univ Hosp, Dept Farm & Lab Clin, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Dept Med Vet, Fac Zootecnia & Engn Alimentos, Sao Paulo, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Experimental Parasitology; v. 157, p. 84-87, OCT 2015. |
| Web of Science Citations: | 6 |
| Abstract | |
Giardia duodenalis is divided into at least eight groups, named assemblages A to H. Assemblages A and B are the only ones able to infect humans and other mammals. The species status for these assemblies is a moot point, but has not gained general acceptance because sexual activity in Giardia is not completely understood. Heterozygosity in G. duodenalis can be detected through simultaneous identification of multiple loci in single cysts or trophozoites. In this paper, we describe a technique that enables simultaneous detection of fragments from four genes from single cysts of G. duodenalis recovered from stool samples. Each cyst from a fecal sample of human origin was separated, the DNA was extracted and amplified by means of multiplex PCR directed to four genes and the multiplex PCR product was further re-amplified using four single PCR (one for each gene). The following loci were detected: beta giardin (bg), GLORF-C4 (orfC4), triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh). This procedure should make it possible to investigate multiple genes from a single cyst of G. duodenalis assemblage A or B. (C) 2015 Elsevier Inc. All rights reserved. (AU) | |
| FAPESP's process: | 11/13472-2 - Genotyping of Giardia duodenalis: detection of mixed infections and genetic recombination in samples of human origin |
| Grantee: | Juliana Martins Aguiar |
| Support Opportunities: | Scholarships in Brazil - Doctorate |