Simultaneous Isolation of Three Different Stem Cell Populations from Murine Skin

Author(s)

Forni, Maria Fernanda ^{[1, 2]} ; Maia Lobba, Aline Ramos ^[2] ; Pereira Ferreira, Alexandre Hamilton ^[2] ; Sogayar, Mari Cleide ^{[2, 1]}

Affiliation

^[1] Univ Sao Paulo, Dept Bioquim, Inst Quim, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Fac Med, Dept Clin Med, Nucleo Terapia Celular & Mol NUCEL NETCEM, BR-05360130 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: PLoS One; v. 10, n. 10 OCT 13 2015.
• Web of Science Citations: 13

Abstract

The skin is a rich source of readily accessible stem cells. The level of plasticity afforded by these cells is becoming increasingly important as the potential of stem cells in Cell Therapy and Regenerative Medicine continues to be explored. Several protocols described single type stem cell isolation from skin; however, none of them afforded simultaneous isolation of more than one population. Herein, we describe the simultaneous isolation and characterization of three stem cell populations from the dermis and epidermis of murine skin, namely Epidermal Stem Cells (EpiSCs), Skin-derived Precursors (SKPs) and Mesenchymal Stem Cells (MSCs). The simultaneous isolation was possible through a simple protocol based on culture selection techniques. These cell populations are shown to be capable of generating chondrocytes, adipocytes, osteocytes, terminally differentiated keratinocytes, neurons and glia, rendering this protocol suitable for the isolation of cells for tissue replenishment and cell based therapies. The advantages of this procedure are far-reaching since the skin is not only the largest organ in the body, but also provides an easily accessible source of stem cells for autologous graft. (AU)

• FAPESP's process: 01/10707-7 - Molecular bases of the control of cell proliferation and origin of neoplasms in the genomic and proteomic era
• Grantee: Mari Cleide Sogayar
• Support type: Research Projects - Thematic Grants