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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Unusual remodeling of the hyalinization band in vulval lichen sclerosus by type V collagen and ECM 1 protein

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Author(s):
Charles A.P. Godoy [1] ; Walcy R. Teodoro [2] ; Ana Paula P. Velosa [3] ; Ana Lucia Garippo [4] ; Esmeralda Miristeni Eher [5] ; Edwin Roger Parra [6] ; Mirian N. Sotto [7] ; Vera L. Capelozzi [8]
Total Authors: 8
Affiliation:
[1] Faculdade de Medicina. Department of Pathology - Brasil
[2] Universidade de São Paulo. Faculdade de Medicina. Division of Rheumatology - Brasil
[3] Universidade de São Paulo. Faculdade de Medicina. Division of Rheumatology - Brasil
[4] Faculdade de Medicina. Department of Pathology - Brasil
[5] Faculdade de Medicina. Department of Pathology - Brasil
[6] Faculdade de Medicina. Department of Pathology - Brasil
[7] Universidade de São Paulo. Faculdade de Medicina. Department of Dermatology - Brasil
[8] Faculdade de Medicina. Department of Pathology - Brasil
Total Affiliations: 8
Document type: Journal article
Source: Clinics; v. 70, n. 5, p. 356-362, 2015-05-00.
Abstract

OBJECTIVES: The vulva is the primary site affected in lichen sclerosus, a chronic dermatosis in women that is histologically characterized by a zone of collagen remodeling in the superior dermis. The normal physiological properties of the vulva depend on the assembly of collagen types I (COLI), III (COLIII) and V (COLV), which form heterotypic fibers, and extracellular matrix protein interactions. COLV regulates the heterotypic fiber diameter, and the preservation of its properties is important for maintaining normal tissue architecture and function. In the current work, we analyzed the expression of COLV and its relationship with COLI, COLIII, elastic fibers and extracellular matrix protein 1 in vulvar biopsies from patients with lichen sclerosus. METHODS: Skin biopsies from 21 patients with lichen sclerosus, classified according to Hewitt histological criteria, were studied and compared to clinically normal vulvar tissue (N=21). Morphology, immunohistochemistry, immunofluorescence, 3D reconstruction and morphometric analysis of COLI, COLIII, COLV deposition, elastic fibers and extracellular matrix 1 expression in a zone of collagen remodeling in the superior dermis were performed. RESULTS: A significant decrease of elastic fibers and extracellular matrix 1 protein was present in the hyalinization zone of lichen sclerosus compared to healthy controls. The non-homogeneous distribution of collagen fibers visualized under immunofluorescence in the hyalinization zone of lichen sclerosus and control skin was confirmed by histomorphometry. Lichen sclerosus dermis shows a significant increase of COLI, COLIII and COLV expression compared to the healthy controls. Significant inverse associations were found between elastic fibers and COLV and between COLV and extracellular matrix 1 expression. A direct association was found between elastic fiber content and extracellular matrix 1 expression. Tridimensional reconstruction of the heterotypic fibers of the lichen sclerosus zone of collagen remodeling confirmed the presence of densely clustered COLV. CONCLUSIONS: Increased deposition of abnormal COLV and its correlation with extracellular matrix 1 and elastic fibers suggest that COLV may be a trigger in the pathogenesis of lichen sclerosus. (AU)

FAPESP's process: 12/03543-2 - Pulmonary fibroblasts in parenchymal remodeling in experimental models of fibrosis induzed by bleomycin and 3-5-di-tert-butyl-4-hydroxytoluene (BHT)
Grantee:Vera Luiza Capelozzi
Support Opportunities: Regular Research Grants
FAPESP's process: 11/52095-0 - Evaluation of the frequency of EGFR, KRAS and EML4-ALK mutation in adenocarcinomas and their subtypes and impact of expression of hyaluronidases and hyaluronic acid synthase in pre-neoplastic lung lesions
Grantee:Vera Luiza Capelozzi
Support Opportunities: Regular Research Grants