| Full text | |
| Author(s): |
da Cunha, Andre Luiz
[1, 2]
;
de Oliveira, Luiz Gustavo
[2]
;
Maia, Lenize Fernandes
[3]
;
Cappa de Oliveira, Luiz Fernando
[3]
;
Michelacci, Yara M.
[1]
;
de Aguiar, Jair Adriano K.
[1, 2]
Total Authors: 6
|
| Affiliation: | [1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Bioquim, BR-04044020 Sao Paulo, SP - Brazil
[2] Univ Fed Juiz de Fora, Inst Ciencias Biol, Dept Bioquim, BR-36036900 Juiz De Fora, MG - Brazil
[3] Univ Fed Juiz de Fora, Inst Ciencias Exatas, Dept Quim, BR-36036900 Juiz de Fora, MG - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Carbohydrate Polymers; v. 134, p. 300-308, DEC 10 2015. |
| Web of Science Citations: | 12 |
| Abstract | |
The aim of the present study was to characterize 16 pharmaceutical grade chondroitin sulfate (CS) samples, concerning the structure and presence of contaminants, in comparison to USP and analytical grade CS. Agarose gel electrophoresis has shown that only 5 samples were >90% CS, while 11 contained less than 15% CS. FACE (fluorophore-assisted carbohydrate electrophoresis) revealed that maltodextrin was the main contaminant in nine of them, and lactose in two. Raman spectroscopy corroborated these results. Concerning the structure of the CS present in the five CS-rich samples, the ratios 4-sulfated:6-sulfated disaccharides varied from 0.9 to 1.7, and their modal molecular weight was 20-29 kDa. Also, they were all contaminated by small amounts of keratan sulfate (<1%). In conclusion, our findings indicate that the composition of CS preparations not always corresponds to the manufacturers' descriptions, and indicate that further characterization should be required for the registry and license of pharmaceutical grade CS. (C) 2015 Elsevier Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 10/16022-5 - Lysosomal enzymes, metalloproteinases and proteoglycans in Diabetes mellitus |
| Grantee: | Yara Maria Corrêa da Silva Michelacci |
| Support Opportunities: | Regular Research Grants |