Bioprospecting and construction of hemicellulases for the production of xylose fro...
| Full text | |
| Author(s): |
Tomazini Junior, Atilio
[1]
;
Dolce, Luciano Graciani
[1]
;
de Oliveira Neto, Mario
[2]
;
Polikarpov, Igor
[1]
Total Authors: 4
|
| Affiliation: | [1] Univ Sao Paulo, Inst Fis Sao Carlos, Dept Fis & Ciencia Interdisciplinar, BR-13560970 Sao Carlos, SP - Brazil
[2] Univ Estadual Paulista, Inst Biociencias, BR-18618970 Botucatu, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | Biotechnology Letters; v. 37, n. 12, p. 2419-2426, DEC 2015. |
| Web of Science Citations: | 4 |
| Abstract | |
Objectives To biochemically characterize an expansin-like X protein domain from Xanthomonas campestris (XcEXLX1) and to study its synergy with cellulases in cellulose depolymerization. Results The protein was purified using a combination of ion exchange and size exclusion chromatography rendering about 30 mg pure protein/l culture medium. Circular dichroism spectroscopy and small-angle X-ray scattering studies of XcEXLX1 reveal that it is a strongly disordered beta-sheet protein. Its low resolution envelope fits nicely the crystallographic structure of the homologous protein EXLX1 from Bacillus subtillis. Furthermore, we demonstrate that XcEXLX1 shows a synergistic, pH-dependent effect when combined with a commercial enzymatic preparation (Accellerase 1500), enhancing its hydrolytic activity on a cellulosic substrate. The strongest effect was observed in acid pHs with an increase in sugar release of up to 36 %. Conclusion The synergistic effect arising from the action of the expansin-like protein was considerable in the presence of significantly larger amounts of the commercial enzymatic cocktail then previously observed (0.35 FPU of Accellerase 1500/g substrate). (AU) | |
| FAPESP's process: | 10/52362-5 - Targeted analysis of microbial lignocellulolytic secretomes: a new approach to enzyme discovery |
| Grantee: | Igor Polikarpov |
| Support type: | Regular Research Grants |
| FAPESP's process: | 09/05349-6 - Expression and purification of cellobiohydrolase I from the filamentous fungus Trichoderma harzianum |
| Grantee: | Bruno Luan Soares Paula de Mello |
| Support type: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 07/08706-9 - Molecular and structural studies of Cellulitic complex enzymes of fungi Trichoderma harzianum with potencial of enzymatic transformation of biomass |
| Grantee: | Viviane Isabel Serpa |
| Support type: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 08/56255-9 - Structure and function of enzymes and auxiliary proteins from Trichoderma, active in cell-wall hydrolysis |
| Grantee: | Igor Polikarpov |
| Support type: | Program for Research on Bioenergy (BIOEN) - Thematic Grants |