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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Transcripts involved in hemostasis: Exploring salivary complexes from Haementeria vizottoi leeches through transcriptomics, phylogenetic studies and structural features

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Xavier Prado Amorim, Adriane Michele [1] ; de Oliveira, Ursula Castro [2] ; Faria, Fernanda [1] ; Mesquita Pasqualoto, Kerly Femanda [1] ; Junqueira-de-Azevedo, Inacio de L. M. [2] ; Chudzinski-Tavassi, Ana Marisa [1, 3]
Total Authors: 6
[1] Inst Butantan, Lab Bioquim & Biofis, BR-05503900 Sao Paulo - Brazil
[2] Inst Butantan, CeTICS, Lab Especial Toxinol Aplicada, BR-05503900 Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Dept Bioquim, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Toxicon; v. 106, p. 20-29, NOV 2015.
Web of Science Citations: 3

Throughout evolution, parasites have adapted in order to successfully intervene in the host defense, producing specific peptides and proteins. Interestingly, these peptides and proteins have been exploited as potential drug candidates against several diseases. Furthermore, biotechnology studies and cDNA libraries have remarkably contributed to identify potentially bioactive molecules. In this regard, herein, a cDNA library of salivary complexes from Haementeria vizottoi leeches was constructed, the transcriptome was characterized and a phylogenetic analysis was performed considering antistasin-like and antiplatelet-like proteins. Hundred twenty three transcripts were identified coding for putative proteins involved in animal feeding (representing about 10% of the expression level). These sequences showed similarities with myohemerythrins, carbonic anhydrases, anticoagulants, antimicrobials, proteases and protease inhibitors. The phylogenetic analysis, regarding antistasin-like and antiplatetlet-like proteins, revealed two main clades in the Rhynchobdellida leeches. As expected, the sequences from H. vizottoi have presented high similarities with those types of proteins. Thus, our findings could be helpful not only to identify new coagulation inhibitors, but also to better understand the biological composition of the salivary complexes. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 11/16393-6 - Transcriptome analysis OF complex salivary LEECH Haementeria vizottoi
Grantee:Adriane Michele Xavier Prado Amorim
Support type: Scholarships in Brazil - Master