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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Mitochondrial DNA dynamics during in vitro culture and pluripotency induction of a bovine Rho0 cell line

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Pessoa, L. V. F. [1] ; Bressan, F. F. [1, 2] ; Chiaratti, M. R. [3, 1] ; Pires, P. R. L. [1] ; Perecin, F. [1, 2] ; Smith, L. C. [1, 4] ; Meirelles, F. V. [1, 2]
Total Authors: 7
[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Cirurgia, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Dept Med Vet, Fac Zootecnia & Engn Alimentos, Pirassununga, SP - Brazil
[3] Univ Fed Sao Carlos, Ctr Ciencias Biol & Saude, Dept Genet & Evolucao, BR-13560 Sao Carlos, SP - Brazil
[4] Univ Montreal, Anim & Reprod Res Ctr, Montreal, PQ - Canada
Total Affiliations: 4
Document type: Journal article
Source: Genetics and Molecular Research; v. 14, n. 4, p. 14093-14104, 2015.
Web of Science Citations: 4

Large number of cellular changes and diseases are related to mutations in the mitochondrial DNA copy number. Cell culture in the presence of ethidium bromide is a known way of depleting mitochondrial DNA and is a useful model for studying such conditions. Interestingly, the morphology of these depleted cells resembles that of pluripotent cells, as they present larger and fragmented mitochondria with poorly developed cristae. Herein, we aimed to study the mechanisms responsible for the control of mitochondrial DNA replication during mitochondrial DNA depletion mediated by ethidium bromide and during the in vitro induction of cellular pluripotency with exogenous transcription factor expression in a bovine model. This article reports the generation of a bovine Rho0 mesenchymal cell line and describes the analysis of mitochondrial DNA copy number in a time-dependent manner. The expression of apoptosis and mitochondrial-related genes in the cells during mitochondrial DNA repletion were also analyzed. The dynamics of mitochondrial DNA during both the depletion process and in vitro reprogramming are discussed. It was possible to obtain bovine mesenchymal cells almost completely depleted of their mitochondrial DNA content (over 90%). However, the production of induced pluripotent stem cells from the transduction of both control and Rho0 bovine mesenchymal cells with human reprograming factors was not successful. (AU)

FAPESP's process: 11/08376-4 - Effects of genetic induction and reprogramming through nuclear transfer in in vitro bovine embryo production
Grantee:Flávio Vieira Meirelles
Support type: Regular Research Grants
FAPESP's process: 10/13384-3 - Effect of introducing embryonic or somatic mitochondria on development and on mitochondrial inheritance in cattle
Grantee:Flávio Vieira Meirelles
Support type: Regular Research Grants
FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
Grantee:Dimas Tadeu Covas
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC