Would calcium or potassium channels be responsible for cardiac arrest produced by adenosine and ATP in the right atria of Wistar rats?

Author(s)

Camara, Henrique ^[1] ; Dantas Rodrigues, Juliano Quintella ^[1] ; Alves, Gabriel Andrade ^[2] ; da Silva Junior, Edilson Dantas ^[1] ; Caricati-Neto, Afonso ^[1] ; Garcia, Antonio G. ^[3] ; Jurkiewicz, Aron ^[1]

Affiliation

^[1] Fed Univ Sao Paulo UNIFESP, Dept Pharmacol, Sao Paulo - Brazil

^[2] Fed Univ Sao Paulo UNIFESP, Dept Biophys, Sao Paulo - Brazil

^[3] Univ Autonoma Madrid, Inst Teofilo Hernando, Madrid - Spain

• Document type: Journal article
• Source: European Journal of Pharmacology; v. 768, p. 199-206, DEC 5 2015.
• Web of Science Citations: 3

Abstract

Autonomic nerves release ATP, which is processed into adenosine in the synaptic cleft. Adenosine and ATP exert a negative chronotropic effect in the heart. This study aims to evaluate adenosine and P2 receptors and cellular signalling in cardiac arrest produced by purines in the heart. Right atria of adult Wistar rats were used to evaluate the effects of adenosine, ATP and CPA (an adenosine A(1) receptor agonist), in the presence and absence of DPCPX, an adenosine A(1) receptor antagonist. Effects of adenosine A(2) and A(3) receptors agonists and antagonists were also investigated. Finally, involvement of calcium and potassium channels in these responses was assessed using BayK 8644 and 4-Aminopyridine. Cumulative concentration-effect curves of adenosine and CPA resulted in a negative chronotropic effect culminating in cardiac arrest at 1000 mu M (adenosine) and 1 mu M (CPA). Furthermore, ATP produced a negative chronotropic effect at 1-300 mu M and cardiac arrest at 1000 mu M in the right atrium. ATP gamma S (a non-hydrolysable analogue of ATP) reduced chronotropism only. The effects of adenosine, CPA and ATP were inhibited by DPCPX, a selective adenosine A(1) receptor antagonist. The selective adenosine A(2) and A(3) receptors antagonists did not alter the chronotropic response of adenosine. 4-Aminopyridine, a blocker of potassium channels at 10 mM, prevented the cardiac arrest produced by adenosine and ATP, while BayK 8644, activator of calcium channels, did not prevent cardiac arrest. Adenosine A(1) receptor activation by adenosine and ATP produces cardiac arrest in the right atrium of Wistar rats predominantly through activation of potassium channels. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 13/20402-6 - Functional, biochemical and molecular study of P1 and P2 purinergic receptors present in the heart from genetically hypertensive rats
• Grantee: Aron Jurkiewicz
• Support Opportunities: Regular Research Grants