| Full text | |
| Author(s): |
Tudela, Renato C.
[1]
;
Loiola, Rodrigo A.
[1]
;
Torres, Tathiany C.
[1]
;
Gil, Noemi L.
[1]
;
Assuncao, Nilson A.
[1]
;
de Noronha, Samuel M. R.
[2]
;
Correa-Noronha, Silvana A.
[2]
;
Landgraf, Richardt G.
[1]
;
Fernandes, Liliam
[1]
Total Authors: 9
|
| Affiliation: | [1] Univ Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, Diadema, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Surg, Escola Paulista Med, Sao Paulo, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | PROTEIN AND PEPTIDE LETTERS; v. 22, n. 12, p. 1111-1116, 2015. |
| Web of Science Citations: | 0 |
| Abstract | |
Kinins are important vasoactive peptides, but the role of the B-1 receptor subtype in the vascular control is poorly understood. This study analyzed the nitric oxide (NO) release, L-arginine (L-Arg) uptake and the expression of the cationic amino acid transporter (CAT) -1 in endothelial cells obtained from B-1 receptor knockout (B-1(-/-)) and wild type (WT) mice. NO production was assessed through a fluorescent dye in living cells stimulated with acetylcholine. L-Arg uptake was determined indirectly in the culture medium by HPLC, in the presence or absence of the CAT-1 blocker N-ethylmaleimide (NEM). CAT-1 mRNA levels and protein expression were determined by qPCR and western blot, respectively. NO release was significantly reduced in B-1(-/-) when compared to WT cells. This result was accompanied by a decreased rate in the L-Arg uptake by B-1(-/-) cells. Incubation with NEM impaired the L-Arg uptake in WT, but had no effect in B-1(-/-) cells. Protein expression and mRNA levels for CAT-1 were reduced in B-1(-/-) in comparison to WT cells. These findings suggest an important role of the endothelial B-1 receptor in the vascular control by interfering with CAT-1 expression, L-Arg uptake and NO release. (AU) | |
| FAPESP's process: | 10/01404-0 - In vivo and in vitro studies of the leptin role in different models of lung inflammation: inflammatory mediators and signaling airways participation |
| Grantee: | Richardt Gama Landgraf |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 14/18760-4 - Venous endothelial factors: influence of angiotensin II and endothelin-1 |
| Grantee: | Liliam Fernandes |
| Support Opportunities: | Regular Research Grants |