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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

DNA methylation analysis of SOCS1, SOCS3, and LINE-1 in microdissected gingival tissue

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Author(s):
Andia, Denise C. [1] ; Planello, Aline C. [1] ; Portinho, Danielle [1] ; da Silva, Rodrigo A. [1] ; Salmon, Cristiane R. [2] ; Sallum, Enilson A. [2] ; Nociti Junior, Francisco H. [2] ; de Souza, Ana P. [1]
Total Authors: 8
Affiliation:
[1] Univ Estadual Campinas, Sch Dent Piracicaba, Div Histol, Dept Morphol, BR-13414018 Sao Paulo, SP - Brazil
[2] Univ Estadual Campinas, Sch Dent Piracicaba, Div Periodont, Dept Prosthodont & Periodont, BR-13414018 Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: CLINICAL ORAL INVESTIGATIONS; v. 19, n. 9, p. 2337-2344, DEC 2015.
Web of Science Citations: 3
Abstract

DNA methylation plays a critical role in the regulation of the transcription of the suppressors of cytokine signaling (SOCS) 1 and SOCS3, which are modulators in the inflammation. We hypothesized that the methylation status of SOCS1, SOCS3, and long interspersed nuclear element (LINE)-1 in gingival tissues previously inflamed would be similar to that found in gingival tissues without clinical inflammation in the period studied. Laser capture microdissection was performed to isolate epithelial and connective gingival tissues. The groups were comprised by ten patients without history of periodontitis and absence of clinical signs of inflammation in the gingiva during the study (healthy group) and ten patients with history of periodontitis, presenting inflammation in the gingival tissue at the first examination of the study (controlled chronic periodontitis group). The gingival biopsies from the controlled chronic periodontitis group were collected after controlling the inflammation. DNA methylation patterns were analyzed using methylation-specific high-resolution melting and combined bisulfite restriction analysis. DNA methylation levels for SOCS1 and SOCS3 did not differ between groups or tissues; likewise, no differences were observed in total LINE-1 methylation or at specific loci. At 3 months following control of inflammation in gingival tissues, the methylation profile of SOCS1, SOCS3, and LINE-1 is similar between connective and epithelial tissues from patients that were previously affected or not by chronic inflammation. Clinical results of a successful treatment are observed after inflammation control and the molecular findings illustrate local and general methylation patterns in recovering tissues toward health conditions and might help to understand events that are occurring in oral cells. (AU)

FAPESP's process: 10/08180-0 - Periodontal inflammation, oxidative and nitrative stress and DNA methylation
Grantee:Ana Paula de Souza
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 10/02338-0 - DNA methylation status of the SOCS1, SOCS2 and SOCS3 genes in the epithelial gingival cells and connective tissue gingival cells in chronic periodontitis.
Grantee:Denise Carleto Andia
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 10/05151-9 - DNA methylation analysis of SOCS1, SOCS2 and SOCS3 gene promoters in oral epithelial cells and gingival tissue cells from individuals with chronic periodontitis: association with gene transcription
Grantee:Aline Cristiane Planello
Support Opportunities: Scholarships in Brazil - Doctorate