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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Differential Viral Fitness Between H1N1 and H3N8 Avian Influenza Viruses Isolated from Mallards (Anas platyrhynchos)

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Author(s):
Ferreira, Helena Lage [1] ; Vangeluwe, Didier [2] ; Van Borm, Steven [3] ; Poncin, Olivier [2] ; Dumont, Nathalie [3] ; Ozhelvaci, Orkun [3] ; Munir, Muhammad [4] ; van den Berg, Thierry [3] ; Lambrecht, Benedicte [3]
Total Authors: 9
Affiliation:
[1] FZEA USP, BR-13635900 Pirassununga, SP - Brazil
[2] Royal Belgian Inst Nat Sci, Belgian Ringing Ctr, B-1000 Brussels - Belgium
[3] CODA CERVA VAR, B-1180 Brussels - Belgium
[4] Pirbright Inst, Woking GU24 0NF, Surrey - England
Total Affiliations: 4
Document type: Journal article
Source: AVIAN DISEASES; v. 59, n. 4, p. 498-507, DEC 2015.
Web of Science Citations: 3
Abstract

Homosubtypic and heterosubtypic immunity in mallards (Anas platyrhynchos) play an important role in the avian influenza virus (AIV) diversity. The mechanisms of AIV replication among wild birds and the role of immunity in AIV diversity have thus not been completely clarified. During the monitoring of AI circulation among wild waterfowl in 2007-2008, two viruses (H3N8 and H1N1) were isolated from ducks caught in a funnel trap located in La Hulpe werland in Belgium. H3N8 viruses were revealed to be more prevalent in the mallard population than was H1N1, which might suggest a better adaptation to this species. In order to investigate this hypothesis, we characterized both isolated viruses biologically by experimental inoculation. Virus excretion and humoral response induced by both isolated viruses were evaluated in mallards after a first infection followed by a homo- or heterosubtypic reinfection under controlled experimental conditions. The H1N1 virus had a delayed peak of excretion of 4 days compared to the H3N8, but the virus shedding was more limited, earlier, and shorter after each reinfection. Moreover, the H3N8 virus could spread to all ducks after homo- or heterosubtypic reinfections and during a longer period. Although the humoral response induced by both viruses after infection and reinfection could be detected efficiently by competitive ELISA, only a minimal H1 antibody response and almost no H3-specific antibodies could be detected by the HI test. Our results suggest that the H3N8 isolate replicates better in mallards under experimental controlled conditions. (AU)

FAPESP's process: 14/23597-5 - 9th International Symposium on Avian Influenza
Grantee:Helena Lage Ferreira
Support Opportunities: Research Grants - Meeting - Abroad