Occurrence of genes coding for MSCRAMM and biofilm-associated protein Bap in Staphylococcus spp. isolated from bovine subclinical mastitis and relationship with somatic cell counts

Author(s):Show less -

Zuniga, Eveline ^[1] ; Melville, Priscilla A. ^[1] ; Saidenberg, Andre B. S. ^[1] ; Laes, Marco A. ^[1] ; Gonsales, Femanda F. ^[1] ; Salaberry, Sandra R. S. ^[1] ; Gregori, Fabio ^[1] ; Brandao, Paulo E. ^[1] ; dos Santos, Franklin G. B. ^[2] ; Lincopan, Nilton E. ^[3] ; Benites, Nilson R. ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Vet Med & Zoothecny, Dept Prevent Vet Med & Anim Hlth, BR-05508270 Sao Paulo - Brazil

^[2] Fed Univ Piaui UFPI, BR-64600000 Picos, Piaui - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil

• Document type: Journal article
• Source: Microbial Pathogenesis; v. 89, p. 1-6, DEC 2015.
• Web of Science Citations: 12

Abstract

This study aimed to elucidate aspects of the epidemiology of bovine subclinical mastitis through the assessment of genes encoding MSCRAMM (microbial surface components recognizing adhesive matrix molecules - a group of adhesins) and protein Bap (implicated in biofilm formation), in coagulase-positive (CPS) and coagulase-negative (CNS) Staphylococcus isolated from subclinical mastitis. Milk samples were collected for microbiological exams, somatic cell count (SCC) and a survey of the genes coding for MSCRAMM (cna, eno, ebpS, fnbA, fnbB and fib) and biofilm-associated protein Bap (bap) in 106 Staphylococcus spp. isolates using PCR. The frequencies of occurrence of eno (82.1%),fnbA (72.6%), fib (71.7%) and bap (56.6%) were higher (P < 0.0001) compared with the other assessed genes (cna, ebpS and fnbB). The higher frequency of occurrence (P < 0.005) of the bap gene in CNS compared with CPS suggests that in these species biofilm formation is an important mechanism for the persistence of the infection. The medians of the SCCs in the samples where eno, fnbA, fib and bap genes were detected were higher compared with Staphylococcus without the assessed genes (P < 0.05) and negative samples (P < 0.01), which indicated that the presence of these MSCRAMM may be related to a higher intensity of the inflammatory process. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 11/51483-6 - Search for adhesins-encoding genes in Staphylococcus spp. isolated from bovine mastitis
• Grantee: Priscilla Anne Melville
• Support Opportunities: Regular Research Grants