| Full text | |
| Author(s): Show less - |
Mendes, Juliano S.
[1]
;
Santiago, Andre da S.
[1]
;
Toledo, Marcelo A. S.
[1]
;
Rosselli-Murai, Luciana K.
[1]
;
Favaro, Marianna T. P.
[1]
;
Santos, Clelton A.
[1]
;
Horta, Maria Augusta C.
[1]
;
Crucello, Aline
[1]
;
Beloti, Lilian L.
[1]
;
Romero, Fabian
[2]
;
Tasic, Ljubica
[2]
;
de Souza, Alessandra A.
[3]
;
de Souza, Anete P.
[4, 1]
Total Authors: 13
|
| Affiliation: | [1] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083875 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Inst Quim, Dept Quim Organ, BR-13083970 Campinas, SP - Brazil
[3] Ctr APTA Citros Sylvio Moreira IAC, BR-13490970 Cordeiropolis, SP - Brazil
[4] Univ Estadual Campinas, Inst Biol, Dept Biol Vegetal, BR-13083862 Campinas, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | PLoS One; v. 10, n. 12 DEC 22 2015. |
| Web of Science Citations: | 4 |
| Abstract | |
Xylella fastidiosa strain 9a5c is a gram-negative phytopathogen that is the causal agent of citrus variegated chlorosis (CVC), a disease that is responsible for economic losses in Brazilian agriculture. The most well-known mechanism of pathogenicity for this bacterial pathogen is xylem vessel occlusion, which results from bacterial movement and the formation of biofilms. The molecular mechanisms underlying the virulence caused by biofilm formation are unknown. Here, we provide evidence showing that virulence-associated protein D in X. fastidiosa (Xf-VapD) is a thermostable protein with ribonuclease activity. Moreover, protein expression analyses in two X. fastidiosa strains, including virulent (Xf9a5c) and nonpathogenic (XfJ1a12) strains, showed that Xf-VapD was expressed during all phases of development in both strains and that increased expression was observed in Xf9a5c during biofilm growth. This study is an important step toward characterizing and improving our understanding of the biological significance of Xf-VapD and its potential functions in the CVC pathosystem. (AU) | |
| FAPESP's process: | 12/51580-4 - Molecular and functional characterization of proteins potentially related to the phytopatogenicity of biofilm-forming bacteria Xylella fastidiosa |
| Grantee: | Anete Pereira de Souza |
| Support Opportunities: | Regular Research Grants |