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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Transcriptome Analysis of Honeybee (Apis Mellifera) Haploid and Diploid Embryos Reveals Early Zygotic Transcription during Cleavage

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Author(s):
Pires, Camilla Valente [1] ; de Paula Freitas, Flavia Cristina [1] ; Cristino, Alexandre S. [2] ; Dearden, Peter K. [3] ; Paulino Simoes, Zila Luz [4]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14049 Ribeirao Preto - Brazil
[2] Univ Queensland, Diamantina Inst, Translat Res Inst, Brisbane, Qld - Australia
[3] Univ Otago, Dept Biochem, Natl Ctr Growth & Dev, Genet Otago & Gravida, Dunedin - New Zealand
[4] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14049 Ribeirao Preto - Brazil
Total Affiliations: 4
Document type: Journal article
Source: PLoS One; v. 11, n. 1 JAN 11 2016.
Web of Science Citations: 9
Abstract

In honeybees, the haplodiploid sex determination system promotes a unique embryogenesis process wherein females develop from fertilized eggs and males develop from unfertilized eggs. However, the developmental strategies of honeybees during early embryogenesis are virtually unknown. Similar to most animals, the honeybee oocytes are supplied with proteins and regulatory elements that support early embryogenesis. As the embryo develops, the zygotic genome is activated and zygotic products gradually replace the preloaded maternal material. The analysis of small RNA and mRNA libraries of mature oocytes and embryos originated from fertilized and unfertilized eggs has allowed us to explore the gene expression dynamics in the first steps of development and during the maternal-to-zygotic transition (MZT). We localized a short sequence motif identified as TAGteam motif and hypothesized to play a similar role in honeybees as in fruit flies, which includes the timing of early zygotic expression (MZT), a function sustained by the presence of the zelda ortholog, which is the main regulator of genome activation. Predicted microRNA (miRNA)-target interactions indicated that there were specific regulators of haploid and diploid embryonic development and an overlap of maternal and zygotic gene expression during the early steps of embryogenesis. Although a number of functions are highly conserved during the early steps of honeybee embryogenesis, the results showed that zygotic genome activation occurs earlier in honeybees than in Drosophila based on the presence of three primary miRNAs (pri-miRNAs) (ame-mir-375, ame-mir-34 and ame-mir-263b) during the cleavage stage in haploid and diploid embryonic development. (AU)

FAPESP's process: 10/08150-3 - Profile of gene expression via regulation of embryonic development males of Apis mellifera
Grantee:Camilla Valente Pires
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 11/03171-5 - Causal analysis of Apis mellifera development: regulatory genes and hierachical networks of gene expression in the specification of tissue and organs
Grantee:Zilá Luz Paulino Simões
Support Opportunities: Research Projects - Thematic Grants