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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Laboratory Performance Predicts the Success of Field Releases in Inbred Lines of the Egg Parasitoid Trichogramma pretiosum (Hymenoptera: Trichogrammatidae)

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Author(s):
Coelho, Jr., Aloisio [1] ; Rugman-Jones, Paul F. [2] ; Reigada, Carolina [1] ; Stouthamer, Richard [2] ; Parra, Jose R. P. [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, ESALQ, Piracicaba, SP - Brazil
[2] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 - USA
Total Affiliations: 2
Document type: Journal article
Source: PLoS One; v. 11, n. 1 JAN 5 2016.
Web of Science Citations: 14
Abstract

In this study we assessed the relationship between the laboratory and field performance of different isofemale lines of Trichogramma pretiosum Riley. In comparative assays, we used three rare mitochondrial haplotypes as genetic markers of the isofemale lines, and by introgressing these mitochondrial haplotypes into each of 15 genetically different nuclear lines, also tested the assumption that mitochondria are neutral markers. In a laboratory trial, 45 isofemale lines (15 nuclear genotypes x three mitochondrial haplotypes) were ranked in three categories (best, intermediate and worst) according to the mean offspring production and the proportion of female offspring. Subsequently, lines from each of the three categories were selected for field releases to quantify field parasitism on Ephestia kuehniella. Temporally separate releases were done in a transgenic Bt cornfield, with four plots, each with 50 points of recapture. The points of recapture consisted of trap cards with eggs of E. kuehniella collected daily. The trap cards were maintained in the laboratory at 25 degrees C until the adult wasps emerged, and the maternal identity of the wasps was determined using qPCR and high-resolution melt curve analysis to determine the mitochondrial haplotype. The results showed that these measures of laboratory performance (fecundity and offspring sex ratio) were good predictors of field success in T. pretiosum. We also report strong evidence discrediting the assumption that mitochondria are neutral, in view of the correlation between performance and mitochondrial haplotype. (AU)

FAPESP's process: 11/17397-5 - GENETIC VARIABILITY OF Trichogramma pretiosum RILEY, 1879, IN ITS ROLE AS A BIOLOGICAL CONTROL AGENT
Grantee:Aloisio Coelho Junior
Support Opportunities: Scholarships in Brazil - Doctorate