Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression

Author(s)

da Silva, Walmir ^[1] ; Souza dos Santos, Robson Augusto ^[2] ; Moraes, Karen C. M. ^[3]

Affiliation

^[1] Univ Fed Ouro Preto, Nucleo Pesquisa Biol, Inst Ciencias Exatas & Biol, Lab Bioquim & Biol Mol, Ouro Preto, MG - Brazil

^[2] Univ Fed Minas Gerais, Dept Fisiol & Biofis, Lab Fisiol, Belo Horizonte, MG - Brazil

^[3] Univ Estadual Paulista, Inst Biociencias, Dept Biol, Mol Biol Lab, BR-13506900 Rio Claro, SP - Brazil

• Document type: Journal article
• Source: Molecular and Cellular Biochemistry; v. 411, n. 1-2, p. 363-371, JAN 2016.
• Web of Science Citations: 6

Abstract

The activated renin-angiotensin-aldosterone system modulates several metabolic pathways that contribute to left ventricular hypertrophy and heart failure. In this metabolic system, angiotensin II modulates heart morphophysiological changes triggered by a series of inflammatory and pro-inflammatory responses; however, the fine tuning associated with the control of this biochemical pathway remains unknown. Here, we investigated elements involved in the post-transcriptional regulation of the pro-inflammatory environment in the H9c2 cardiac cell line, focusing on miRNA elements that modulate PTEN expression. A cellular model of investigation was established and the miR-315-5p was identified as a novel element targeting PTEN in this cardiac cell line, thereby controlling the protein level. This interconnected pathway contributes to the control of the pro-inflammatory environment in Ang II-treated cells. (AU)

• FAPESP's process: 09/07671-2 - Addressing mechanistic questions on the induction process of cardiac hypertrophy mediated by the inflammation process: functional studies of CUGBP2 protein
• Grantee: Karen Cristiane Martinez de Moraes
• Support Opportunities: Regular Research Grants