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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Skin Delivery and in Vitro Biological Evaluation of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles for Skin Disorder Therapies

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Author(s):
Rigon, Roberta B. [1] ; Fachinetti, Naiara [1] ; Severino, Patricia [2] ; Santana, Maria H. A. [3] ; Chorilli, Marlus [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Paulista, UNESP, Fac Ciencias Farmaceut, Dept Farmacos & Med, Campus Araraquara, BR-14800850 Araraquara, SP - Brazil
[2] Univ Tiradentes, Ctr Ciencias Biol & Saude, BR-49010390 Aracaju, SE - Brazil
[3] Univ Estadual Campinas, Fac Engn Quim, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Molecules; v. 21, n. 1 JAN 2016.
Web of Science Citations: 16
Abstract

The aim of this study was to evaluate the skin delivery and in vitro biological activity of trans-resveratrol (RES)-loaded solid lipid nanoparticles (SLNs). The SLNs were composed of stearic acid, poloxamer 407, soy phosphatidylcholine (SPC), an aqueous phase and 0.1% RES. The particle size, polydispersity index (PdI) and zeta potential were analyzed by dynamic light scattering (DLS). The SLNs were analyzed by scanning electron microscopy (SEM-FEG) and differential scanning calorimetry (DSC). In vitro RES-SLN skin permeation/retention assays were conducted, and their tyrosinase inhibitory activity was evaluated. An MTT reduction assay was performed on HaCat keratinocytes to determine in vitro cytotoxicity. The formulations had average diameter lower than 200 nm, the addition of SPC promoted increases in PdI in the RES-SLNs, but decreases PdI in the RES-free SLNs and the formulations exhibited zeta potentials smaller than -3 mV. The DSC analysis of the SLNs showed no endothermic peak attributable to RES. Microscopic analysis suggests that the materials formed had nanometric size distribution. Up to 45% of the RES permeated through the skin after 24 h. The RES-loaded SLNs were more effective than kojic acid at inhibiting tyrosinase and proved to be non-toxic in HaCat keratinocytes. The results suggest that the investigated RES-loaded SLNs have potential use in skin disorder therapies. (AU)

FAPESP's process: 12/19568-4 - Evaluation of the potential of nanostructured lipid systems for cutaneous administration of trans-resveratrol
Grantee:Marlus Chorilli
Support Opportunities: Regular Research Grants
FAPESP's process: 11/16888-5 - Development and characterization of solid lipid nanoparticles for dermal administration of trans-resveratrol
Grantee:Roberta Balansin Rigon
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 13/21500-1 - Solid lipid nanoparticles for incorporation of trans-resveratrol in the topical treatment of melanoma: in vitro and in vivo evaluation and the elucidation of the mechanism of uptake cellular
Grantee:Roberta Balansin Rigon
Support Opportunities: Scholarships in Brazil - Doctorate