Classification of thermophilic actinobacteria isolated from arid desert soils, including the description of Amycolatopsis deserti sp nov.

Author(s)

Busarakam, Kanungnid ^{[1, 2]} ; Brown, Ros ^[1] ; Bull, Alan T. ^[3] ; Tan, Geok Yuan Annie ^[4] ; Zucchi, Tiago D. ^{[5, 6]} ; da Silva, Leonardo Jose ^[5] ; de Souza, Wallace Rafael ^[5] ; Goodfellow, Michael ^[1]

Affiliation

^[1] Newcastle Univ, Sch Biol, Newcastle Upon Tyne NE1 7RU, Tyne & Wear - England

^[2] Thailand Inst Sci & Technol Res, Dept Agr Technol, Pathum Thani 12120 - Thailand

^[3] Univ Kent, Sch Biosci, Canterbury CT2 7NJ, Kent - England

^[4] Univ Malaya, Inst Biol Sci, Kuala Lumpur 50603 - Malaysia

^[5] EMBRAPA Meio Ambiente, Microbiol Lab, Rod SP 340-Km 127, BR-13820000 Jaguariuna - Brazil

^[6] Agrivalle, Av Tranquillo Giannini 1050, BR-13329600 Salto - Brazil

• Document type: Journal article
• Source: ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY; v. 109, n. 2, p. 319-334, FEB 2016.
• Web of Science Citations: 7

Abstract

The taxonomic position of 26 filamentous actinobacteria isolated from a hyper-arid Atacama Desert soil and 2 from an arid Australian composite soil was established using a polyphasic approach. All of the isolates gave the diagnostic amplification product using 16S rRNA oligonucleotide primers specific for the genus Amycolatopsis. Representative isolates had chemotaxonomic and morphological properties typical of members of the genus Amycolatopsis. 16S rRNA gene analyses showed that all of the isolates belong to the Amycolatopsis methanolica 16S rRNA gene clade. The Atacama Desert isolates were assigned to one or other of two recognised species, namely Amycolatopsis ruanii and Amycolatopsis thermalba, based on 16S rRNA gene sequence, DNA:DNA relatedness and phenotypic data; emended descriptions are given for these species. In contrast, the two strains from the arid Australian composite soil, isolates GY024(T) and GY142, formed a distinct branch at the periphery of the A. methanolica 16S rRNA phyletic line, a taxon that was supported by all of the tree-making algorithms and by a 100 % bootstrap value. These strains shared a high degree of DNA:DNA relatedness and have many phenotypic properties in common, some of which distinguished them from all of the constituent species classified in the A. methanolica 16S rRNA clade. Isolates GY024(T) and GY142 merit recognition as a new species within the A. methanolica group of thermophilic strains. The name proposed for the new species is Amycolatopsis deserti sp. nov.; the type strain is GY024(T) (=NCIMB 14972(T) = NRRL B-65266(T)). (AU)

• FAPESP's process: 11/14333-6 - Diversity and polyphasic taxonomy of actinobacteria isolated from reforestation areas
• Grantee: Tiago Domingues Zucchi
• Support Opportunities: Scholarships in Brazil - BIOTA - Young Researchers